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Rapid field testing of field water samples for total coliform and E. coli using isothermal amplification and unique micro-sized particles for cell capture and concentration

Award Information
Agency: Department of Defense
Branch: Defense Health Agency
Contract: W81XWH-17-C-0112
Agency Tracking Number: H171-004-0145
Amount: $149,841.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: DHA17-004
Solicitation Number: 2017.1
Solicitation Year: 2017
Award Year: 2017
Award Start Date (Proposal Award Date): 2017-08-15
Award End Date (Contract End Date): 2018-03-14
Small Business Information
2415 N. Triphammer Rd
Ithaca, NY 14850
United States
DUNS: 824968477
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Aaron Strickland
 VP of Research and Development
 (607) 227-7522
Business Contact
 Gregory Mouchka
Phone: (607) 229-5322
Research Institution

iFyber LLC proposes to develop a coliform assay with differential detection of E. coli that will circumvent the current challenges with detecting these organisms at a level of 1 CFU/mL in less than 16 hours. The proposed assay targets a result in less than 6 hours by making use of novel microsphere reagents for capture and concentration of the target cells, and coupling these reagents with loop-mediated amplification (LAMP) of coliform and E. coli genetic markers with fluorescence detection. Microspheres will be used to concentrate the target cells by at least 1000 fold, where they will then be further enriched with growth medium, and their respective genetic reporters will be subsequently amplified through LAMP. This PhaseI effort will lay the foundation for this concept with a breadboard device, which will be refined in Phase II for commercial development.

* Information listed above is at the time of submission. *

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