Soil Inhibition Resistant Mutants of Taq DNA Polymerase

Award Information
Agency:
Department of Agriculture
Amount:
$290,400.00
Program:
SBIR
Contract:
2006-33610-17604
Solitcitation Year:
N/A
Solicitation Number:
N/A
Branch:
N/A
Award Year:
2006
Phase:
Phase II
Agency Tracking Number:
2005-00194
Solicitation Topic Code:
N/A
Small Business Information
DNA Polymerase Technology, Inc.
1508 South Grand Blvd., St. Louis, MO, 63104
Hubzone Owned:
N
Woman Owned:
N
Socially and Economically Disadvantaged:
N
Duns:
N/A
Principal Investigator
 Milko Kermekchiev
 (314) 771-5566
 milko@klentaq.com
Business Contact
 Wayne Barnes
Title: President
Phone: (314) 680-0575
Email: wayne@klentaq.com
Research Institution
N/A
Abstract
Gene detection in soil using the Polymerase Chain Reaction (PCR) is nearly impossible due to the inhibitory affects of the components of soil, including humic acid. Currently, the DNA must be extracted from the soil in order to perform PCR. The DNA extraction/purification step is expensive and time consuming. We intend to develop commercially available PCR enzymes that will enable PCR to detect pathogens directly in soil, eliminating the extraction/purification step entirely.

* information listed above is at the time of submission.

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