Soil Inhibition Resistant Mutants of Taq DNA Polymerase
Department of Agriculture
Agency Tracking Number:
Solicitation Topic Code:
Small Business Information
DNA Polymerase Technology, Inc.
1508 South Grand Blvd., St. Louis, MO, 63104
Socially and Economically Disadvantaged:
AbstractGene detection in soil using the Polymerase Chain Reaction (PCR) is nearly impossible due to the inhibitory affects of the components of soil, including humic acid. Currently, the DNA must be extracted from the soil in order to perform PCR. The DNA extraction/purification step is expensive and time consuming. We intend to develop commercially available PCR enzymes that will enable PCR to detect pathogens directly in soil, eliminating the extraction/purification step entirely.
* information listed above is at the time of submission.