Data Driven Sequence Assembly

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$75,000.00
Award Year:
1994
Program:
SBIR
Phase:
Phase I
Contract:
1 R43 GM51680-1,
Agency Tracking Number:
25116
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Dnastar, Inc.
1228 S Park Street, Madison, WI, 53715
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Schuyler Baldwin
(608) 258-7420
Business Contact:
() -
Research Institution:
n/a
Abstract
This grant will improve the software for DNA sequencing by integrating the components of basecalling, sequence assembly and post-assembly analysis into an integrated software system. To test theperformance of the software configurations, data for known regions of E. coli will be resequenced fromthe original clones using a LI-Cor sequencing instrument. The data will be basecalled by neural net basedpattern recognition and assembled with a variety of multiple alignment methods. Algorithmic solutionswill be compared and evaluated relative to the goal of achieving an accurate final sequence with theminimum of editing by a human expert. Alleviating the need for sequence editing will present theopportunity for significant cost savings in genome projects and other research involving DNA sequencing.

* information listed above is at the time of submission.

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