Data Driven Sequence Assembly

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 R43 GM51680-1,
Agency Tracking Number: 25116
Amount: $75,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1994
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Dnastar, Inc.
1228 S Park Street, Madison, WI, 53715
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Schuyler Baldwin
 (608) 258-7420
Business Contact
Phone: () -
Research Institution
N/A
Abstract
This grant will improve the software for DNA sequencing by integrating the components of basecalling, sequence assembly and post-assembly analysis into an integrated software system. To test theperformance of the software configurations, data for known regions of E. coli will be resequenced fromthe original clones using a LI-Cor sequencing instrument. The data will be basecalled by neural net basedpattern recognition and assembled with a variety of multiple alignment methods. Algorithmic solutionswill be compared and evaluated relative to the goal of achieving an accurate final sequence with theminimum of editing by a human expert. Alleviating the need for sequence editing will present theopportunity for significant cost savings in genome projects and other research involving DNA sequencing.

* information listed above is at the time of submission.

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