HIGH-THROUGHPUT FLUORESCENT PROTEIN KINASE ASSAYS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,110.00
Award Year:
2002
Program:
SBIR
Phase:
Phase I
Contract:
1R43GM066439-01
Award Id:
61033
Agency Tracking Number:
GM066439
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
ECHELON BIOSCIENCES, INC., 420 CHIPETA WAY, STE 180, SALT LAKE CITY, UT, 84108
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
MICHAEL MOSTERT
(801) 588-0455
MMOSTERT@ECHELON-INC.COM
Business Contact:
THOMAS HEATH
(801) 588-0455
GTHEATH@ECHELON-INC.COM
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): Protein kinases catalyze the phosphorylation of serine (Ser), threonine (Thr), and tyrosine (Tyr) residues in peptides and proteins, and comprise the largest superfamily of enzymes in eukaryotes with an estimated 500 genes in the human genome. Because most intracellular processes are regulated by protein phosphorylation, dysregulation of protein kinases can lead to a variety of disease states. Protein kinases are therefore important targets for drug discovery and development. Protein kinase-targeted drug discovery requires the ability to rapidly screen compounds for their ability to specifically inhibit selected protein kinases. To facilitate the high-throughput screening of kinase activities we will develop microplate protein kinase assays using a proprietary fluorescence technology developed at the University of Utah. First, we will prepare two sets of fluorescently labeled synthetic peptides. One set will be used to assay protein Ser/Thr kinases, the other for protein Tyr kinases. Second, we will optimize each protein kinase assay system for use in standard 96-well and 384-well fluorescence microplate readers. Based on feedback from outside evaluators, we will optimize and develop commercial versions of these protein kinase assay kits to be sold to the research community and companies involved in drug discovery and development.

* information listed above is at the time of submission.

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