Fluorogenic Assays for Phospholipid Detection

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43GM067365-01
Agency Tracking Number: GM067365
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2003
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
ECHELON BIOSCIENCES, INC., 675 ARAPEEN DR, STE 302, SALT LAKE CITY, UT, 84108
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 LI FENG
 (801) 588-0455
 LFENG@ECHELON-INC.COM
Business Contact
 JOHN WIRTHLIN
Phone: (801) 588-0455
Email: JWIRTHLIN@ECHELON-INC.COM
Research Institution
N/A
Abstract
DESCRIPTION (provided by applicant): Phospholipids (PL) including phosphoinositides (PIPn) are crucial structural and signaling molecules for proper inter- and intra-cellular communication in an organism. The disruption of lipid homeostasis is associated with numerous human pathologies. High levels of LPA, LPC, PI (3,4,5) P3, PI (3) P and PI (4,5) P2 are linked to specific pathologies such as cancer, myotubular myopathy and Lowe's syndrome. The inter-conversions of phospholipids are mediated by the interplay of phospholipases, kinases, phosphatases and other lipid binding proteins. It is important to analyze and quantify cellular and extra-cellular phospholipids to measure enzyme activities associated with specific pathologies. We propose to develop a fluorogenic assay based on chemically or genetically modified proteins that selectively recognize PLs and PIPns. The modification will be site-specific and will incorporate novel light sensitive entities. These reagents will be applied in Phase II to develop high throughput screening methods for rapid and automated assay of lipid kinases and phosphatases. Such reagents can also be used in the development of cell-based assays.

* Information listed above is at the time of submission. *

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