Hybrid Phospholipid-Phosphoinositides

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$100,152.00
Award Year:
2003
Program:
SBIR
Phase:
Phase I
Contract:
1R43GM069074-01
Award Id:
66521
Agency Tracking Number:
GM069074
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
ECHELON BIOSCIENCES, INC., 675 ARAPEEN DR, STE 302, SALT LAKE CITY, UT, 84108
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
PAUL NEILSEN
(801) 588-0455
PNEILSEN@ECHELON-INC.COM
Business Contact:
(801) 588-0455
Research Institution:
n/a
Abstract
DESCRIPTION (provided by applicant): Phosphoinositide recognition by binding proteins and lipid-metabolizing enzymes involves specific interactions with the head group and diacylglycerol backbone, which vary significantly from protein to protein. When a fluorescent probe is introduced into the inositol head group, binding and metabolism are severely attenuated or abrogated entirely. When a fluorescent probe is introduced into the acyl groups, many of the resulting derivatives fail to show adequate Km and Vmax values as substrates for lipid kinases and phosphatases as well as binding to cognate PIP-binding proteins. Recently, Echelon Biosciences has developed a new approach to make phospholipid-phophoinositide hybrids based on a four-carbon threitol backbone. These new materials, known as Pea-PIPs TM, have a phosphatidylethanolamine head group that permits biochemical or chemical probes to be attached in a position spatially orthogonal to the phosphoinositide head group and the two acyl chains. In this Phase i grant, we will first synthesize a full repertoire of all eight Pea-PIPns TM. Second, we will prepare two Pea-PIPs TM with four different hydrophilic linker arms to optimize probe-head group distances. Third, we will establish the feasibility of using Pea- PIPs TM as in vitro substrates for lipid kinases, lipid phosphatases, and lipid binding proteins.

* information listed above is at the time of submission.

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