Fluorogenic Assays for Measuring the Activity of Ceramidase and Sphingomyelinase

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1R43GM084502-01
Agency Tracking Number: GM084502
Amount: $100,000.00
Phase: Phase I
Program: SBIR
Awards Year: 2008
Solicitation Year: 2008
Solicitation Topic Code: N/A
Solicitation Number: PHS2007-2
Small Business Information
DUNS: 179151188
HUBZone Owned: Y
Woman Owned: Y
Socially and Economically Disadvantaged: Y
Principal Investigator
 () -
Business Contact
Phone: (801) 588-0455
Email: ksauder@echelon-inc.com
Research Institution
DESCRIPTION (provided by applicant): Sphingolipids are a class of lipids that have important roles as structural components of cell membranes yet are also key signaling molecules involved in numerous cellular processes such as apoptosis, cell survival, dif ferentiation, motility, and growth. The levels of the various sphingolipids are kept in balance by metabolizing enzymes and disruptions by abnormal levels of these enzymes are known to occur in diseases such as cancer, diabetes, arthersclerosis, and autoim mune disorders. In this application, fluorogenic assays for two important sphingolipid metabolizing enzymes, ceramidase and sphingomyelinase, will be developed. The fluorescence dequenching assays utilize internally quenched fluorescent substrates which, w hen hydrolyzed by the enzymes, fluoresce due to the quencher no longer being in close proximity to the fluor. Labeled analogs of ceramide and sphingomyelin will be synthesized, evaluated as substrates with their respective enzymes, and the assay conditions will be optimized. These assays can be employed to further basic research and be adapted to high throughput screening for therapeutic compounds. PUBLIC HEALTH RELEVANCE: The overall goal of this project is to develop assays for two important lipid metabolizing enzymes: ceramidase and sphingomyelinase. These assays will help to further basic research in this growing field and potentially be used for developing new drugs for diseases such as cancer, diabetes, and Alzheimer's disease.

* information listed above is at the time of submission.

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