THE PROPOSED PROGRAM RELATES TO THE SYNTHESIS OF (13)C MULTILABELED CHOLESTEROL AND -SITOSTEROL AS POTENTIAL DIAGNOSTIC AGENTS.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 1538
Amount: $49,979.00
Phase: Phase I
Program: SBIR
Awards Year: 1984
Solitcitation Year: N/A
Solitcitation Topic Code: N/A
Solitcitation Number: N/A
Small Business Information
Tracer Technologies Inc.
225 Needham Street, Newton, MA, 02164
Duns: N/A
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 SUN SHINE YUAN
 PRINCIPAL SCIENTIST
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE PROPOSED PROGRAM RELATES TO THE SYNTHESIS OF (13)C MULTILABELED CHOLESTEROL AND -SITOSTEROL AS POTENTIAL DIAGNOSTIC AGENTS. HYPERCHOLESTEROLEMIA HAS BEEN IMPLICATED AS A PRIMARY RISK FACTOR IN CORONARY HEART DISEASE YET MAJOR QUESTIONS REGARDING CHOLESTEROL ABSORPTION, TRANSPORT, REVERSE TRANSPORT AND DISPOSITION REMAIN TO BE ELUCIDATED FOR LACK OF A SAFE, CONVENIENT NON-RADIOACTIVE TRACER METHODOLOGY. THUS, STABLE ISOTOPE LABELED DIETARY STEROLS IN CLINICAL PROTOCOLS, COMBINED WITH MASS FRAGMENTOGRAPHY, CAN BE EXPECTED TO AID THE ELABORATION OF INDECES FOR SUCH PARAMETERS AS STEROL POOL SIZE, TRANSIT TIME, VOLUME DISTRIBUTION AND CLEARANCE. THE SYNTHETIC APPROACH REQUIRES DISCONNECTION AT C-22 OF THE STEROL SIDE CHAIN AND RESYNTHESIS WITH THE LABELS POSITIONED IN THE ISOPROPYL TERMINUS AT (C-25, 26 AND 27), THE LATTER BEING DERIVED FROM (13)C3-ACETONE. INCLUDED AMONG THE PROJECT TASKS ARE DEVELOPMENT OF PATHWAYS CONSERVATIVE TOWARDS ISOTOPE AND OPTIMIZATION OF YIELDS TO PROVIDE MULTIGRAM QUANTITIES OF BOTH STEROLS IN SEVEN SYNTHETIC STEPS. YIELDS IN THE 10-20% RANGE ARE EXPECTED. ADDITIONALLY, THE PROGRAM CALLS FOR DEVELOPMENT OF AN A-RING DEGRADATION AND RESYNTHESIS VIA THE ROBINSON ANNELATION TO GIVE (13)C4-LABELED STEROLS. A REPERTORY OF VARIOUS INCREMENTALLY LABELED A AND/OR CHAIN LABELED STEROLS CAN THEN BE ENVISIONED FOR APPLICATION IN SIMULTANEOUS STUDIES BY MULTIPLE ROUTES OF TRACER ADMINISTRATION.

* information listed above is at the time of submission.

Agency Micro-sites

US Flag An Official Website of the United States Government