NOVEL CLONING AND TRANSFER SYSTEMS FOR BACILLUS THURINGIENSIS INSECTICIDAL GENES

Award Information
Agency:
National Science Foundation
Branch
n/a
Amount:
$199,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
2630
Agency Tracking Number:
2630
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
2005 Cabot Blvd W, Langhorne, PA, 19047
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Bruce C Carlton Phd
Vice Pres Res & Dev
(215) 757-1590
Business Contact:
() -
Research Institution:
n/a
Abstract
THE PRINCIPAL OBJECTIVES OF THIS PROPOSAL ARE (1) TO EVALUATE A NOVEL BACILLUS HOST-VECTOR SYSTEM FOR THE CLONINGAND EXPRESSION OF GENES SPECIFYING MICROBIAL INSECTICIDAL ACTIVITIES; AND (2) TO DETERMINE THE FEASIBILITY OF AN INTERGENIC CONJUGAL TRANSFER SYSTEM FOR MOBILIZING RECOMBINANT PLASMIDS BETWEEN A VARIETY OF GRAM-POSITIVE HOSTS. A DELTA-ENDOTOXIN GENE FROM A PROTOTYPE STRAIN OF BACILLUS THURINGEIENSIS VAR. KURSTAKI (HD-73) WILL BE CLONED INTO THEPLASMID VECTOR PBD9, AND TRANSFORMED INTO A PLASMID-FREE STRAIN OF B. MEGATERIUM. RECOMBINANT CLONES CONTAINING THE TOXIN GENE WILL BE IDENTIFIED EITHER BY MICROSCOPIC EXAMINATION OF SPORULATED COLONIES FOR VISIBLE CRYSTALS OR BY COLONY HYBRIDIZATION. THESE RECOMBINANT PLASMIDS WILL BEEVALUATED FOR TRANSFERABILITY BETWEEN VARIOUS STRAINS OF BACILLUS AND STREPTOCOCCUS, USING WELL-CHARACTERIZED TRANSMISSIBLE PLASMIDS AS THE MOBILIZING PLASMID. THESE APPROACHES WILL ULTIMATELY ENABLE THE CONSTRUCTION OF NOVEL COMBINATIONS OF INSECTICIDAL GENES IN HOST BACTERIA THAT AREMAXIMIZED FOR FERMENTATION YIELDS.

* information listed above is at the time of submission.

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