NOVEL CLONING AND TRANSFER SYSTEMS FOR BACILLUS THURINGIENSIS INSECTICIDAL GENES

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: N/A
Agency Tracking Number: 2630
Amount: $199,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1987
Solitcitation Year: N/A
Solitcitation Topic Code: N/A
Solitcitation Number: N/A
Small Business Information
Ecogen Inc.
2005 Cabot Blvd W, Langhorne, PA, 19047
Duns: N/A
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Bruce C Carlton Phd
 Vice Pres Res & Dev
 (215) 757-1590
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE PRINCIPAL OBJECTIVES OF THIS PROPOSAL ARE (1) TO EVALUATE A NOVEL BACILLUS HOST-VECTOR SYSTEM FOR THE CLONINGAND EXPRESSION OF GENES SPECIFYING MICROBIAL INSECTICIDAL ACTIVITIES; AND (2) TO DETERMINE THE FEASIBILITY OF AN INTERGENIC CONJUGAL TRANSFER SYSTEM FOR MOBILIZING RECOMBINANT PLASMIDS BETWEEN A VARIETY OF GRAM-POSITIVE HOSTS. A DELTA-ENDOTOXIN GENE FROM A PROTOTYPE STRAIN OF BACILLUS THURINGEIENSIS VAR. KURSTAKI (HD-73) WILL BE CLONED INTO THEPLASMID VECTOR PBD9, AND TRANSFORMED INTO A PLASMID-FREE STRAIN OF B. MEGATERIUM. RECOMBINANT CLONES CONTAINING THE TOXIN GENE WILL BE IDENTIFIED EITHER BY MICROSCOPIC EXAMINATION OF SPORULATED COLONIES FOR VISIBLE CRYSTALS OR BY COLONY HYBRIDIZATION. THESE RECOMBINANT PLASMIDS WILL BEEVALUATED FOR TRANSFERABILITY BETWEEN VARIOUS STRAINS OF BACILLUS AND STREPTOCOCCUS, USING WELL-CHARACTERIZED TRANSMISSIBLE PLASMIDS AS THE MOBILIZING PLASMID. THESE APPROACHES WILL ULTIMATELY ENABLE THE CONSTRUCTION OF NOVEL COMBINATIONS OF INSECTICIDAL GENES IN HOST BACTERIA THAT AREMAXIMIZED FOR FERMENTATION YIELDS.

* information listed above is at the time of submission.

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