A FIBEROPTIC PROBE FOR IN VIVO NEUROTRANSMITTER ANALYSIS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 14044
Amount: $49,974.00
Phase: Phase I
Program: SBIR
Awards Year: 1990
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
111 Downey St, Norwood, MA, 02062
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Lois S Robblee
 (617) 769-9450
Business Contact
Phone: () -
Research Institution
N/A
Abstract
MICRODIALYSIS (MD) IS AN INCREASINGLY IMPORTANT TOOL FOR SAMPLING THE EXTRACELLULAR FLUID OF SPECIFIC REGIONS OF THE BRAIN. REAL-TIME, DIRECT ANALYSIS OF THE MICRODIALYSATEWOULD BE ADVANTAGEOUS FOR STUDYING DYNAMIC CHANGES IN BRAIN CHEMISTRY AS A FUNCTION OF BEHAVIOR OR IN RESPONSE TO EXPERIMENTAL PARADIGMS OR TRAUMA. THE RESEARCH WILL DEVELOP A SURFACE-ENHANCED RAMAN SPECTROSCOPY (SERS) PROBE ON THE TIP OF AN OPTIC FIBER IN DIMENSIONS SUITABLE FOR INSERTION INTO AN IN VIVO MICRODIALYSIS CANNULA. THE SERS/MD PROBE WILL ENABLE IN VIVO ANALYSIS OF NEUROTRANSMITTERS OR OTHER SUBSTANCES IN BRAIN EXTRACELLULAR FLUID BY SERS TECHNIQUES. THE PHASE I OBJECTIVES ARE TO FABRICATE OPTICAL FIBERS WITH SERS SUBSTRATES, COUPLE THEM WITH MICRODIALYSIS CANNULAS, AND DEMONSTRATE DOPAMINE ANALYSIS DURING MICRODIALYSIS FROM A SIMULATED BRAIN EXTRACELLULAR FLUID. SUCCESSFUL DEMONSTRATION OF THE FEASIBILITY OF A SERS/MD PROBE WILL PROVIDE A FOUNDATION FOR PHASE II WORK TO EVALUATE THE PROBEDURING IN VIVO MICRODIALYSIS EXPERIMENTS AND TO DEVELOP A PROTOTYPE SERS/MD PROBE AND INSTRUMENTATION PACKAGE FOR USE IN ACADEMIC, CLINICAL, AND PHARMACEUTICAL NEUROCHEMISTRY LABORATORIES.

* Information listed above is at the time of submission. *

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