A FIBEROPTIC PROBE FOR IN VIVO NEUROTRANSMITTER ANALYSIS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$49,974.00
Award Year:
1990
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
14044
Agency Tracking Number:
14044
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
111 Downey St, Norwood, MA, 02062
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Lois S Robblee
(617) 769-9450
Business Contact:
() -
Research Institute:
n/a
Abstract
MICRODIALYSIS (MD) IS AN INCREASINGLY IMPORTANT TOOL FOR SAMPLING THE EXTRACELLULAR FLUID OF SPECIFIC REGIONS OF THE BRAIN. REAL-TIME, DIRECT ANALYSIS OF THE MICRODIALYSATEWOULD BE ADVANTAGEOUS FOR STUDYING DYNAMIC CHANGES IN BRAIN CHEMISTRY AS A FUNCTION OF BEHAVIOR OR IN RESPONSE TO EXPERIMENTAL PARADIGMS OR TRAUMA. THE RESEARCH WILL DEVELOP A SURFACE-ENHANCED RAMAN SPECTROSCOPY (SERS) PROBE ON THE TIP OF AN OPTIC FIBER IN DIMENSIONS SUITABLE FOR INSERTION INTO AN IN VIVO MICRODIALYSIS CANNULA. THE SERS/MD PROBE WILL ENABLE IN VIVO ANALYSIS OF NEUROTRANSMITTERS OR OTHER SUBSTANCES IN BRAIN EXTRACELLULAR FLUID BY SERS TECHNIQUES. THE PHASE I OBJECTIVES ARE TO FABRICATE OPTICAL FIBERS WITH SERS SUBSTRATES, COUPLE THEM WITH MICRODIALYSIS CANNULAS, AND DEMONSTRATE DOPAMINE ANALYSIS DURING MICRODIALYSIS FROM A SIMULATED BRAIN EXTRACELLULAR FLUID. SUCCESSFUL DEMONSTRATION OF THE FEASIBILITY OF A SERS/MD PROBE WILL PROVIDE A FOUNDATION FOR PHASE II WORK TO EVALUATE THE PROBEDURING IN VIVO MICRODIALYSIS EXPERIMENTS AND TO DEVELOP A PROTOTYPE SERS/MD PROBE AND INSTRUMENTATION PACKAGE FOR USE IN ACADEMIC, CLINICAL, AND PHARMACEUTICAL NEUROCHEMISTRY LABORATORIES.

* information listed above is at the time of submission.

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