ELCATECH HAS DEVELOPED A NEW TECHNIQUE FOR THE IMMUNOASSAY OF CLOTTING FACTORS.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
4976
Agency Tracking Number:
4976
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
4291 Lantern Drive, Winston-salem, NC, 27106
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
GEORGE J DOELLGAST
PRINCIPAL INVESTIGATOR
(919) 748-4219
Business Contact:
() -
Research Institute:
n/a
Abstract
ELCATECH HAS DEVELOPED A NEW TECHNIQUE FOR THE IMMUNOASSAY OF CLOTTING FACTORS. THE TECHNIQUE IS SIMILAR TO THE ELISA METHODOLOGY IN THAT ENZYME-LABELED DERIVATIVES OF ANTIGENS AND ANTIBODIES ARE USED TO DETECT IMMUNE COMPLEXES. IT DIFFERS FROM MOST ELISA METHODS IN THAT IT USES AN ACTIVATOROF COAGULATION (RUSSELL'S VIPER VENOM FACTOR X ACTIVATOR OR RVV-XA) AS THE LABELING ENZYME AND A UNIQUE CLOTTING ASSAY, ENTITLED ENZYME-LINKED COAGULATION ASSAY (ELCA), AS THE DETECTION SYSTEM. ELCA USES PEROXIDASE-LABELED FIBRINOGEN IN SOLUTION AND UNLABELED FIBRINOGEN TOGETHER AS A SUBSTRATEFOR THROMBIN, WHICH IS GENERATED IN THE CLOTTING CASCADE. THROMBIN CLEAVES BOTH FORMS TO FIBRIN AND CONSEQUENTLY PEROXIDASE-FIBRIN IS BOUND TO THE SOLID PHASE. USED AS A DETECTION SYSTEM FOR RVV-XA, THIS ASSAY CAN MEASURE AS LITTLE AS 50 FEMTOGRAMS/ML OF RVV-XA, WHICH IS MORE THAN THREE ORDERS OF MAGNITUDE LOWER THAN THE DETECTION LIMIT FORPEROXIDASE. ANTIGENS AND ANTIBODIES CAN BE LABELED WITH THIS ENZYME; THESE CONJUGATES CAN THEN BE USED FOR COMPET- ITIVE IMMUNOASSAYS WITH HIGH SENSITIVITY. BY COMBINATION OFTHE SPECIFICITY OF MONOCLONAL ANTIBODIES FOR FACTORS OF INTEREST (II,VII,IX,X,AND PROTEIN C) WITH THIS SENSITIVE DETECTION SYSTEM, RESEARCHERS WILL ASSAY THREE IMPORTANT PARAMETERS OF INTEREST IN THROMBOSIS AND HEMOSTASIS, THAT IS, ACTIVATED FACTOR X, CLOTTING FACTOR ANTIGEN LEVELS, AND COMPLEXES OF FACTOR XA WITH ANTITHROMBIN III. THESE IMMUNO-ASSAYS, IN CONJUNCTION WITH THE SENSITIVE FUNCTIONAL DETECT-ION OF CLOTTING FACTORS BY ELCA, WILL PROVIDE A COMPLETE SYSTEM FOR THE DETECTION OF COMPONENTS OF INTEREST IN HEMOSTASIS AND THROMBOSIS.

* information listed above is at the time of submission.

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