A UNIQUE ENZYME LINKED FIBRINOLYTIC ASSAY SYSTEM

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$500,000.00
Award Year:
1989
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
8055
Agency Tracking Number:
8055
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
4291 Lantern Dr, Winston-salem, NC, 27106
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
() -
Research Institute:
n/a
Abstract
THROMBOLYTIC THERAPY HAS BECOME AN INCREASINGLY IMPORTANT TOOL IN THE TREATMENT OF ACUTE MYOCARDIAL INFARCTION. THE MOST RECENTLY APPROVED THERAPEUTIC AGENT FOR THIS PURPOSE IST-PA (TISSUE PLASMINOGEN ACTIVATOR) WHICH HAS THE ADVANTAGESOF BEING FIBRIN SPECIFIC AND IS RAPIDLY CLEARED IN VIVO BY THE LIVER. THE DETECTION AND QUANTITATION OF THIS COMPONENTOF THE FIBRINOLYTIC SYSTEM AS WELL AS ITS INHIBITORS IS STILL TIME CONSUMING AND IS MEASURED BY LYSIS ZONE AREAS. WE PROPOSE A NEW TECHNIQUE FOR THE QUANTITATION OF ALL THE COMPONENTS OF THE FIBRINOLYTIC SYSTEM INCLUDING T-PA, PLASMINOGEN, PLASMIN, ALPHA(2)-ANTIPLASMIN, AND ANTIACTIVATORS (EG PAI-1). THE ASSAY USES A SOLID PHASE ENZYME LABELLED FIBRIN AS A SUBSTRATE FOR LOCALLY GENERATED PLASMIN. THE MANIPULATION OF CONCENTRATIONS OF ACTIVATORS AND INHIBITORS WILL DIRECTLY INFLUENCE THE AMOUNT OF PLASMIN PRODUCED. QUANTITATION OF THE RESULTING PLASMIN IS ACHIEVED THROUGH THE DETECTION AND MEASUREMENT OF ENZYME LABELLED FIBRIN FRAGMENTS RELEASED THROUGH THE PROTEOLYTIC ACTION OF THE PLASMIN. THIS ASSAY TECHNIQUE HAS A NUMBER OFADVANTAGES OVER EXISTING ASSAYS INCLUDING THE USE OF SOLID PHASE FIBRIN AS A SUBSTRATE AND RATE ENHANCER WHICH PARALLELS THE SITUATION IN VIVO. THE ASSAY IS ALSO CONVENIENT FOR HANDLING MULTIPLE SAMPLES, LENDS ITSELF TO AUTOMATION AND BECAUSE OF ITS SENSITIVITY IS EXTREMELY COST EFFECTIVE.

* information listed above is at the time of submission.

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