ENZYMES THAT ARE L-GLUTAMINASES EXHIBIT ANTITUMOR ACTIVITY.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
4741
Agency Tracking Number:
4741
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
3760 Haven Avenue, Menlo Park, CA, 94025
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
CAROL A TALKINGTON
PRINCIPAL INVESTIGATOR
(415) 324-8600
Business Contact:
() -
Research Institute:
n/a
Abstract
ENZYMES THAT ARE L-GLUTAMINASES EXHIBIT ANTITUMOR ACTIVITY. THUS, WORK TO DEVELOP THESE ENZYMES INTO THERAPEUTIC AGENTS IS IN PROGRESS. PRESENT LIMITATIONS INCLUDE THE ENZYMES' SUBSTRATE AFFINITY, SIZE, IMMUNOGENICITY, AND, IN SOME CASES, DUAL FUNCTION AS BOTH L-GLUTAMINASE AND L- ASPARAGINASE. THE OBJECTIVE OF THIS PROJECT IS TO OBTAIN A SINGLE-FUNCTION L-GLUTAMINASE THAT IS SMALLER IN SIZE. PHASE I INCLUDES BOTH THE CLONING AND EXPRESSION OF THE GENEENCODING PSEUDOMONAS 7A L-GLUTAMINASE-L-ASPARAGINASE, AN ENZYME WITH VERY HIGH AFFINITY FOR L-GLUTAMINE. NOT ONLY WILL PHASE I WORK PROVIDE A LARGER AND CHEAPER SOURCE OF ENZYME FOR CLINICAL TRIALS, BUT IT WILL ALSO ALLOW THE PURSUIT OF THE SECOND PHASE OF THE PROJECT. PHASE II WILL OBTAIN, THROUGH OLIGONUCLEOTIDE AND DELETION MUTAGENESIS, ANENZYME THAT IS EXCLUSIVELY AN L-GLUTAMINASE AND THAT IS SUFFICIENTLY SMALL TO ALLOW FOR IMPROVED PENETRABILITY OF TUMORS LOCATED IN THE EXTRAVASCULAR SPACE. THE ANTITUMOR ACTIVITY OF THE RESULTANT GENETICALLY-ENGINEERED ENZYMES WILL BE TESTED BY INJECTING THEM INTO TUMOR-BEARING MICE. DEPENDING UPON THE SIMILARITIES BETWEEN PROKARYOTIC AND EUKARYOTIC L-GLUTAMINASES, THE CLONED PSEUDOMONAS 7A GENE MIGHT ALSO SERVE AS A PROBE TO ISOLATE A HUMAN, NONIMMUNO- GENIC, L-GLUTAMINASE.

* information listed above is at the time of submission.

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