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Rapid field testing of field water samples for total coliform and E. coli using isothermal amplification and unique micron-sized particles for cell capture and concentration

Award Information
Agency: Department of Defense
Branch: Defense Health Agency
Contract: W81XWH18C0148
Agency Tracking Number: H2-0348
Amount: $999,285.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: DHA17-004
Solicitation Number: 17.1
Solicitation Year: 2017
Award Year: 2020
Award Start Date (Proposal Award Date): 2020-09-24
Award End Date (Contract End Date): 2023-01-23
Small Business Information
2415 N. Triphammer Rd
Ithaca, NY 14850
United States
DUNS: 824968477
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Aaron Strickland
 VP of Research and Development
 () -
Business Contact
 Greg Mouchka
Phone: (607) 330-2307
Research Institution

iFyberLLC proposes to develop acoliform assay with differential detection of E.coliand total coliform that will circumvent thecurrent challenges with detectingthese organismsatalevel of1 CFU per100mLin less than 8 hours. The proposed assay targetsaresult in less than 8 hours by making use of novel microspherereagents forcaptureand concentration of thetargetcells,and couplingthesereagents with loopmediated amplification (LAMP) ofcoliform and E.coligeneticmarkers with fluorescence detection.Microspheres will be used to concentratethe targetcells byat least1000fold,wherethey will then befurtherenriched with growth medium,and their respectivegenetictargets will be subsequentlyamplified through LAMP. This PhaseIIeffort is building offasuccessful PhaseI project that showed feasibility for theconcept. PhaseII will befocused on assay optimization,kit development,and development ofaalpha prototypesystem that will bevalidated next to currentlyapproved EPA ATP methods.

* Information listed above is at the time of submission. *

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