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Rapid field testing of field water samples for total coliform and E. coli using isothermal amplification and unique micron-sized particles for cell capture and concentration

Award Information
Agency: Department of Defense
Branch: Defense Health Program
Contract: W81XWH18C0148
Agency Tracking Number: H2-0348
Amount: $999,825.00
Phase: Phase II
Program: SBIR
Solicitation Topic Code: DHA17-004
Solicitation Number: 2017.1
Solicitation Year: 2017
Award Year: 2018
Award Start Date (Proposal Award Date): 2018-08-27
Award End Date (Contract End Date): 2021-01-26
Small Business Information
2415 N. Triphammer Rd, Ithaca, NY, 14850
DUNS: 824968477
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Aaron Strickland
 () -
Business Contact
 Greg Mouchka
Phone: (607) 229-5322
Research Institution
iFyber LLC proposes to develop a coliform assay with differential detection of E. coli and total coliform that will circumvent the current challenges with detecting these organisms at a level of 1 CFU per 100 mL in less than 8 hours. The proposed assay targets a result in less than 8 hours by making use of novel microsphere reagents for capture and concentration of the target cells, and coupling these reagents with loop-mediated amplification (LAMP) of coliform and E.coli genetic markers with fluorescence detection. Microspheres will be used to concentrate the target cells by at least 1000 fold, where they will then be further enriched with growth medium, and their respective genetic targets will be subsequently amplified through LAMP. This Phase II effort is building off a successful Phase I project that showed feasibility for the concept. Phase II will be focused on assay optimization, kit development, and development of a alpha prototype system that will be validated next to currently approved EPA ATP methods.

* Information listed above is at the time of submission. *

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