A METHOD TO DEVELOP A MODEL SYSTEM FOR NONRADIOACTIVE DNA HYBRIDIZATION/DETECTION FOR IDENTIFICATION OF BACTERIA WILL BE DEVELOPED.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 1350
Amount: $36,832.00
Phase: Phase I
Program: SBIR
Awards Year: 1984
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Enzo Biochem Inc
325 Hudson Street, New York, NY, 10013
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 HUEY LANG YANG
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
A METHOD TO DEVELOP A MODEL SYSTEM FOR NONRADIOACTIVE DNA HYBRIDIZATION/DETECTION FOR IDENTIFICATION OF BACTERIA WILL BE DEVELOPED. DNA FOR AMPICILLIN RESISTANCE WILL BE LABELED WITH BIOTINYLATED DEOXYURIDINE TRIPHOSPHATE BY NICK TRANSLATION AND USED AS PROBE. COLONY HYBRIDIZATION PROCEDURES FOR THE DETECTION OF IN E. COLI WILL THEN BE DEVELOPED. DETECTION OF HYBRIDIZED BIOTINYLATED DNA WILL BE DONE BY DETECTION OF BIOTIN BY STREPTAVIDIN, A BIOTIN BINDING PROTEIN LINKED TO A COLOR PRODUCING ENZYME. ONCE THE SYSTEM IS ESTABLISHED, ATTEMPTS WILL BE MADE TO DETECT SEQUENCES OF LOWER COPY NUMBER AND OF SHORTER LENGTH. ATTEMPTS WILL BE MADE TO SHORTEN THE TIME REQUIRED TO PERFORM THE PROCEDURE. THIS MODEL SYSTEM IS BEING DEVELOPED AS A BASIS FOR ADAPTATION TO DIRECT (NON-GROWTH) TESTS FOR BACTERIAL PATHOGEN-CONTAINING CLINICAL SAMPLES. POTENTIAL COMMERCIAL SIGNIFICANCE THAT COULD BE REALIZED FROM DEVELOPMENT OF THIS TECHNOLOGY IS FAST AND CONVENIENT DIAGNOSIS AND CHARACTERIZATION OF BACTERIAL INFECTIONS.

* information listed above is at the time of submission.

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