DETECTION OF AIDS VIRUS (HTLV-III) BY MONOCLONAL ANTIBODIES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$406,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
4978
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Epitope Inc.
15425-e Southwest Koll Parkway, Beaverton, OR, 97006
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Denis R Burger
Principal Investigator
(503) 641-6115
Business Contact:
() -
Research Institution:
n/a
Abstract
THE OBJECTIVE OF THIS PROPOSAL IS TO USE MOUSE MONOCLONAL ANTIBODIES TO HTLV-III TO EVALUATE THE FEASIBILITY OF DETECTING HTLV ANTIGEN EXPRESSION ON T LYMPHOCYTES IN CLINICAL SPECIMENS FROM PATIENTS WITH LYPHADENOPATHY SYNDROME AND OVERT AIDS, AND IN INDIVIDUALS IN HIGH RISK GROUPS. THIS WILL BE DONE BY: (1) PRODUCING AN ARRAY OF MOUSE MONOCLONALS TO HTLV-III ANTIGENS BY IMMUNIZING WITH SELECTED ANTIGENS TO EXTENSIVE IMMUNIZATION PROTOCOLS; (2) SELECTING HYBRID CULTURES REACTIVE TO HTLV-III BY ELISA SCREENING AND WESTERN BLOTTING AND TO CLONE AND CHARACTERIZEHYBRIDS WHICH EXPRESS SELECTIVE REACTIVITY PATTERNS TO HTLV-III; (3) TO EVALUATE SELECTED MONOCLONAL ANTIBODIES FOR USE IN DETECTING HTLV-III ANTIGEN EXPRESSION ON HUMAN T LYMPHO- CYTES BY FLOW CYTOMETRY; AND (4) TO USE SELECTED MONOCLONAL ANTIBODIES IN A RETROSPECTIVE TRIAL TO COMPARE (A) ELISA REACTIVITY, (B) WESTERN BLOTTING, (C) T LYMPHOCYTE H/S RATIO, AND D HTLV ANTIGEN EXPRESSION BY FLOW CYTOMETRY ON A BANK OF SELECTED SAMPLES.

* information listed above is at the time of submission.

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