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STTR Phase I: DNA Assembly for Directed Expression of Industrial Enzymes Using a Novel Hyperthermophilic Genome

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: 0611274
Agency Tracking Number: 0611274
Amount: $100,000.00
Phase: Phase I
Program: STTR
Solicitation Topic Code: BT
Solicitation Number: NSF 05-605
Solicitation Year: 2006
Award Year: 2006
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
301 Sparkman Drive
Huntsville, AL 35899
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Damien Marsic
 (256) 348-2769
Business Contact
 Joseph Ng
Phone: (256) 824-3715
Research Institution
 Univ of AL Huntsville
 Joseph D Ng
301 Sparkman Drive
Huntsville, AL 35805
United States

 (256) 824-3715
 Nonprofit College or University

This Small Technology Transfer Research (STTR) Phase I project aims to exploit a newly sequenced genome of a hyperthermophilic microorganism for the production of thermal stable enzymes that are useful for molecular engineering and industrial application. The targeted DNA coding regions can be prepared for recombinant protein expression without extended manipulations in restriction digest and ligation reactions. Small-scale production of targeted gene products can be quickly evaluated for solubility and stability to determine potential scale-up production. The procedure is most attractive for future studies to implement point mutations, create chimeric enzymes and perform domain shuffling for optimizing enzyme functionality. The impact of the proposal is of significant commercial value as well as having social impact in production new biocatalytic protein that may be more robust and thermal stable for industrial processes, including detergents, textile, food processing, medical applications and energy. Moreover, novel enzymes that are more active and effective for drug intermediates would be immensely useful for biotransformation in the pharmaceutical industry and consequently affect world health in general.

* Information listed above is at the time of submission. *

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