A Portable Vibrio Cholerae Concentrator for Sensitive Pathogen Detection in Water

Award Information
Agency: Department of Commerce
Branch: National Institute of Standards and Technology
Contract: 70NANB19H040
Agency Tracking Number: 054-FY19-63
Amount: $99,999.48
Phase: Phase I
Program: SBIR
Solicitation Topic Code: None
Solicitation Number: N/A
Timeline
Solicitation Year: 2019
Award Year: 2019
Award Start Date (Proposal Award Date): 2019-08-01
Award End Date (Contract End Date): 2020-01-31
Small Business Information
2042 Mailbu Dr., West Lafayette, IN, 47906
DUNS: 080918577
HUBZone Owned: N
Woman Owned: Y
Socially and Economically Disadvantaged: N
Principal Investigator
 Katherine Clayton
 (415) 309-9524
 kclayton@omnivistech.com
Business Contact
 Katherine Clayton
Phone: (415) 309-9524
Email: kclayton@omnivistech.com
Research Institution
N/A
Abstract
This SBIR Phase I project proposes to develop an easy to use, inexpensive, and portable bacterial concentrator to enable more sensitive cholera pathogen (Vibrio cholerae) detection. Cholera affects communities across 41 countries, including Mozambique in 2019 after Cyclone Idai and Yemen in 2017. Current methods used to detect the cholera pathogen in water involves a 3 to 5-day procedure due to the low concentrations of the bacterium found in the water. The proposed device intakes 1L of water from an environmental water source and concentrates the solution down to 1mL, enriching the bacteria contained within the sample. Therefore, the enriched sample can be used in conjunction with our handheld Vibrio cholerae detection device to identify low, but dangerous, bacterial levels. This project first proposes the development of an easy-to-use portable water detection device. We will evaluate the concentrator’s usability after construction. Secondly, we will test the capture and concentration efficiency of the device by filtering and measuring varying concentrations of polystyrene fluorescent particles that pass through the filter. Lastly, we will determine the capture efficiency of Vibrio cholerae spiked environmental water samples. The final step will provide critical knowledge toward both the ability to enrich for Vibrio cholerae and the degree of which fouling of the filter will take place.

* Information listed above is at the time of submission. *

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