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Delivery of nematicides to control plant parasitic nematodes by using Tobacco mild green mosaic tobamovirus as a nanocarrier

Award Information
Agency: Department of Agriculture
Branch: N/A
Contract: 2019-01022
Agency Tracking Number: 2019-01022
Amount: $99,957.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: 8.2
Solicitation Number: USDA-NIFA-SBIR-006649
Timeline
Solicitation Year: 2019
Award Year: 2019
Award Start Date (Proposal Award Date): 2019-09-10
Award End Date (Contract End Date): 2020-04-30
Small Business Information
3131 NW 13th Street Suite 54, Gainesville, FL, 32609-2183
DUNS: 134355341
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Raghavan Charudattan
 President & CEO
 (352) 278-1572
 rcharudattan@bioprodex.com
Business Contact
 Raghavan Charudattan
Title: President & CEO
Phone: (352) 278-1572
Email: rcharudattan@bioprodex.com
Research Institution
N/A
Abstract
We have developed a bioinspired nanoparticle technology using the coat protein (CP) assemblies ofTobacco mild green mosaic tobamovirus (TMGMV) to deliver nematicides to roots reduce dosageand improve soil mobility efficacy and safety. The CP's zwitterionic nature allows the loading andrelease of nematicides. The TMGMV-delivered nematicides (TDN) are efficacious againstCaenorhabditis elegans in liquid cultures and the TMGMV-assisted delivery enhances thenematicide's soil mobility compared to the free nematicide and contemporary syntheticnanoparticles. With this foundation we will use experimental and computational approaches tooptimize nematicide payloads in TMGMV and assess the efficacy of TDN in vitro and in soil.Abamectin azadirachtin and fluensulfone will be studied tailoring their loading and release byusing counterions and covalent chemistries. The effective concentration (EC50) of TDN vs. freenematicides will be compared against C. elegans. Soil behavior will be modeled to predict thediffusion profiles of TDN and validated with soil leaching experiments considering soil grain sizepH temperature and organic matter concentration. The loading dose and application rate requiredto treat crops in different settings will be determined from studying the efficacy of TDN vs. freenematicides using potted tomato plants infested with Meloidogyne incognita.Besides proof of concept and feasibility of delivering registered commercially used nematicidesrather than model compounds in Phase I we will establish the applicability of TMGMV as apesticide delivery platform to target plant and environmental pests. Phase II objectives will aim todevelop test and gather registration data and design an industrial process to mass-produce thedesired TDN formulations. In Phase III commercial products will be launched.

* Information listed above is at the time of submission. *

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