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Antibody Fragments to Improve HIV gp120 Protein Production

Award Information
Agency: Department of Health and Human Services
Branch: National Institutes of Health
Contract: 75N93019C00009
Agency Tracking Number: N43AI190009
Amount: $299,815.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: NIAID
Solicitation Number: N/A
Solicitation Year: 2018
Award Year: 2019
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
321 Jones Boulevard
POTTSTOWN, PA 19464-3468
United States
DUNS: 962964990
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Hiep Tran
 (610) 990-7531
Business Contact
 Hiep Tran
Phone: (610) 990-7531
Research Institution

Camelid single domain antibody fragmentsVHHpossess a number of distinctive features including a small sizean elevated stabilityand an ability to recognize epitopes non accessible for conventional antibodiesBesides common research and immunotherapy applicationscamelid VHHs are also used as co crystallization chaperonesprotein aggregation controllers and enzyme activity tunersIn this proposalwe wish to utilize these unique VHH properties as a chaperone for HIV gpexpressionfolding and stability to improve gpprotein productionIn Phase Icamelid VHH antibodies to HIV gpwill be developed that are capable of enhancing gpexpression in yeast and in mammalian cells enabling a simple mode for rapid and scalable affinity purificationAt least afold improvement in gpproduction in mammalian cell transient expression will be the basis for phase II transitionThe effort in Phase II will be to develop stable VHH assisted gpprotein production CHO cell lines with significant improvement in yieldGMP manufacturing cell line development and Master Cell bank generation will be performedFollowing developmentscale up of upstream and downstream GMP processes will be completed

* Information listed above is at the time of submission. *

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