NEW APPROACHES TO PRODUCTION OF HLA TYPING REAGENTS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$495,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
1352
Agency Tracking Number:
1352
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1055 Lemon Street, Menlo Park, CA, 94025
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Chih Ping Liu
Senior Scientist
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
HUMAN CLASS II HISTOCOMPATIBILITY ANTIGEN TYPING IS ALREADY OF SUBSTANTIAL IMPORTANCE IN DETERMING DISEASE SUSCEPTIBILITY IN INDIVIDUALS. PRESENT SEROLOGIC METHODS OF CLASS II ANTIGEN TYPING ARE LIMITED BY THE POLYCLONAL NATURE OF MANY ANTISERA, BY THE FACT THAT MANY OF THE AVAILABLE HUMAN ANTISERA REACT WITH MULTIPLE DIFFERENT DETERMINANTS AND BY THE DIFFICULTY OF OBTAINING ALLELE SPECIFIC AS COMPARED WITH SPECIES SPECIFIC MURINE MONOCLONAL ANTISERA AGAINST HUMAN DR CLASS II MHC ANTIGENS. THE PRESENT PROPOSAL IS TO DEVELOP A PROCEDURE THAT WILL RAPIDLY MAKE IT POSSIBLE TO CLONE AND EXPRESS COMPOSITE CLASS II ANTIGENS THAT HAVE SINGLE EXONS FROM HUMAN DC, DR OR SB ANTIGENS INCORPORATED AND SUBSTITUTING FOR CORRESPONDING EXONS OF ANALOGOUS MOUSE ANTIGENS. THESE WILL THEN BE USED FOR IMMUNIZATION PROCEDURES TO GENERATE MOUSE MONOCLONAL ANTIBODIES, A MUCH LARGER FRACTION OF WHICH WILL BE ALLELE SPECIFIC RATHER THAN SPECIES SPECIFIC. THEREFORE, THESE PROCEDURES COULD LEAD TO THE EFFICIENT PRODUCTION OF A NEW GENERATION OF SEROLOGIC REAGENTS FOR HUMAN MHC TYPING THAT WOULD PROVIDE MUCH BETTER RESOLUTION THAN THE PRESENTLY AVAILABLE REAGENTS.

* information listed above is at the time of submission.

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