MONOCLONAL ANTIBODIES TO HTLV-III/LAV

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4795
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1986
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
871 Industrial Road, Building J, San Carlos, CA, 94070
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 KOU HWANG
 PRINCIPAL INVESTIGATOR
 (415) 594-1600
Business Contact
Phone: () -
Research Institution
N/A
Abstract
MOUSE AND RAT MONOCLONAL ANTIBODIES WILL BE PRODUCED AGAINSTHTLV-III, THE HUMAN RETROVIRUS ASSOCIATED WITH AIDS AND A SPECTRUM OF RELATED SYNDROMES. GENE LABS WILL EMPLOY INTACTHTLV-III VIRIONS COUPLED TO KLH AS AN IMMUNOGEN AND USE A SENSITIVE ELISA ASSAY, BASED ON PURIFIED HTLV-III AS THE ANTIGEN IN OUR PRIMARY SCREENING PROCEDURE. IMMUNOPRECIPI- TATION, WESTERN BLOTTING, AND CELLULAR IMMUNOFLUORESCENCE WILL BE USED TO CHARACTERIZE STABLE, IMMUNOGLOBULIN- SECRETING, ELISA-REACTIVE SUBCLONES. ANTIBODY BINDING CONSTANTS WILL BE ASSESSED BY STANDARD PROCEDURES. FINE MAPPING OF ANTIBODY SPECIFICITY AS IT CORRELATES WITH BIOLOGICAL EFFECTS WILL BE EXAMINED IN THE CONTEXT OF SEPARATE PHASE II FUNDING. PRELIMINARY STUDIES HAVE DEMONSTRATED THE FEASIBILITY OF THE PROPOSAL APPROACH, RESULTING IN THE GENERATION OF BOTH MOUSE AND RAT MONOCLONALANTIBODIES TO HTLV-III DETERMINED ANTIGENS. THE SUCCESSFUL COMPLETION OF THIS PROJECT WILL INVOLVE THE ESTABLISHMENT OFA COMPLETE PANEL OF RAT AND MOUSE MONOCLONAL ANTIBODIES WITHHIGH AVIDITY AGAINST EPITOPES THAT MAY BE WEAKLY IMMUNOGENICOR SILENT IN THE NORMAL HOST.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government