DIPAIN-Based Handheld Assay for the Detection of T-2 Toxin in Water Using a Handheld Reader

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$99,939.00
Award Year:
2010
Program:
STTR
Phase:
Phase I
Contract:
W911SR-10-C-0062
Award Id:
94909
Agency Tracking Number:
A10A-021-0354
Solicitation Year:
n/a
Solicitation Topic Code:
ARMY 10T021
Solicitation Number:
n/a
Small Business Information
824 Interchange Boulevard, Newark, DE, 19711
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
115451549
Principal Investigator:
YliRemo Vallejo
VP of R&D
(302) 283-1730
yli@anptinc.com
Business Contact:
RobertDaniel
Sr. VP of Government Contracts
(302) 283-1730
robert@anptinc.com
Research Institute:
University of California, Irvine
Zhibin Guan
Department of Chemistry
1102 Natural Sciences 2
Irvine, CA, 92697
(949) 824-5172

Abstract
A rapid assay for the detection of T2 trichothecene mycotoxin in water is proposed that will use ANP Technology, Inc.'s established handheld reader and rapid acetylcholinesterase (AChE) inhibitor test ticket format. Dipain-II and other candidate Dipain derivatives will be immobilized on cellulose and other solid supports mounted as discs in wells on the existing test ticket, similar to the AChE inhibitor ticket which uses a cellulose support for an enzyme substrate. The fluorescent signal emitted when the association complex of Dipain-II and T2 is formed can be measured by ANP's handheld reader by changing the light source to an LED at or near 366 nm output. The current camera onboard can measure the resultant yellow orange emission. A time to result of ~10 seconds with a sensitivity to as low as 50 ng/mL is expected. The ticket will use a built in comparator so that it is self-calibrating.

* information listed above is at the time of submission.

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