DIPAIN-Based Handheld Assay for the Detection of T-2 Toxin in Water Using a Handheld Reader

Award Information
Agency: Department of Defense
Branch: Army
Contract: W911SR-10-C-0062
Agency Tracking Number: A10A-021-0354
Amount: $99,939.00
Phase: Phase I
Program: STTR
Awards Year: 2010
Solicitation Year: 2010
Solicitation Topic Code: A10A-T021
Solicitation Number: 2010.A
Small Business Information
824 Interchange Boulevard, Newark, DE, 19711
DUNS: 115451549
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Yli Remo Vallejo
 VP of R&D
 (302) 283-1730
 yli@anptinc.com
Business Contact
 Robert Daniel
Title: Sr. VP of Government Contracts
Phone: (302) 283-1730
Email: robert@anptinc.com
Research Institution
 University of California, Irvine
 Zhibin Guan
 Department of Chemistry
1102 Natural Sciences 2
Irvine, CA, 92697
 (949) 824-5172
 Nonprofit college or university
Abstract
A rapid assay for the detection of T2 trichothecene mycotoxin in water is proposed that will use ANP Technology, Inc.'s established handheld reader and rapid acetylcholinesterase (AChE) inhibitor test ticket format. Dipain-II and other candidate Dipain derivatives will be immobilized on cellulose and other solid supports mounted as discs in wells on the existing test ticket, similar to the AChE inhibitor ticket which uses a cellulose support for an enzyme substrate. The fluorescent signal emitted when the association complex of Dipain-II and T2 is formed can be measured by ANP's handheld reader by changing the light source to an LED at or near 366 nm output. The current camera onboard can measure the resultant yellow orange emission. A time to result of ~10 seconds with a sensitivity to as low as 50 ng/mL is expected. The ticket will use a built in comparator so that it is self-calibrating.

* Information listed above is at the time of submission. *

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