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IDENTIFICATION AND ISOLATION OF THE CYSTIC FIBROSIS GENE

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 7323
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Timeline
Solicitation Year: N/A
Award Year: 1987
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
871 Industrial Road Building J
San Carlos, CA 94070
United States
DUNS: N/A
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 KIRK E FRY PHD
 (415) 594-1600
Business Contact
Phone: () -
Research Institution
N/A
Abstract

COMPLETE AND PARTIAL RESTRICTION-ENZYME DIGESTS OF GENOMIC DNA WILL BE ANALYZED BY PFGE TO MAP THE KNOWN DNA MARKERS LINKED TO THE GENE FOR CYSTIC FIBROSIS. THESE TECHNIQUES WILL BE USED TO IDENTIFY LARGE DNA FRAGMENTS THAT SPAN THE CHROMOSOMAL SITE OF THE CYSTIC FIBROSIS GENE. THESE FRAGMENTS WILL BE USED TO PREPARE CDNA LIBRARIES CORRESPONDING TO ALL MRNAS ENCODED BY DNA OF THE SIZE OF THE FRAGMENT. FURTHER SCREENING OF THE CDNA LIBRARIES WILL BE PERFORMED TO ISOLATE THOSE CLONES THAT ARE ENCODED ON CHROMOSOME 7. EACH SUCH CLONE FROM CYSTIC FIBROSIS, NORMAL PANCREATIC, AND NASAL POLYP TISSUE WILL BE COMPARED. POTENTIAL MUTATIONS WILL BE IDENTIFIED, AND DNA FROM A GROUP OF DISEASED SUBJECTS AND PHENOTYPICALLY NORMAL INDIVIDUALS WILLBE EVALUATED TO CONFIRM THE ASSOCIATION OF THE MUTATION WITHTHE DISEASE.

* Information listed above is at the time of submission. *

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