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Development of a Cell Culture System for HIV Infection

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 R43 AI36770-1,
Agency Tracking Number: 24801
Amount: $78,960.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 1994
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
505 Penobscot Drive
Redwood City, CA 94063
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Albert Tam
 (415) 369-9500
Business Contact
Phone: () -
Research Institution

Hepatitis E virus (HEV) is the major etiologic agent for enterically transmitted non-A, non-Bhepatitis. Hepatitis E represents more than half of the acute viral hepatitis cases occurring in developingcountries where large epidemics affect young to middle-aged adults. Systematic studies on HEVreplication and pathogenesis are needed to guide diagnosis and prevention. No current method isavailable to grow HEV in culture. Our goal is to identify an in vitro infectious HEV molecule and toestablish a cell culture system amenable to supporting viral replication. A full-length cDNA clone andin vitro-synthesized RNA transcripts of HEV will be tested for their respective abilities to infectsusceptible cells in culture. To confirm in vitro infection, we will examine RNA- and DNA-transfectedcells for the presence of replicative RNA species as well as authentic viral proteins. With theestablishment of a cell culture system for HEV infection, we will, in future phase studies, investigate itspotential utilities to provide useful reagents for the development of diagnostics and vaccine. It will alsoprovide a model to study HEV cellular pathogenesis and, thus, potential ways to interfere with theprocess.

* Information listed above is at the time of submission. *

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