HAMSTER OVARY CELL CHROMOSOMAL ABERRATION ASSAY FOR EXAMINING INDIRECT MECHANISMS OF MUTAGENS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
4909
Agency Tracking Number:
4909
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Genesys Research Inc (Currently Genesys Research, Inc.)
2224 Old Middlefield Way, Suite C, Mountain View, CA, 94043
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
ANN D MITCHELL
PRINCIPAL INVESTIGATOR
(415) 968-3534
Business Contact:
() -
Research Institution:
n/a
Abstract
IN VITRO CYTOGENETIC TESTS TO EVALUATE THE GENETIC HAZARDS OF CHEMICALS ARE INCREASING IN IMPORTANCE FOR PREDICTING IN VIVO EFFECTS. IN PARTICULAR, STUDIES HAVE DEMONSTRATED (1) THE RELEVANCE OF CHROMOSOMAL MUTATION TO INHERITANCE IN GERMCELLS; (2) THAT SPECIFIC CHROMOSOMAL DEFECTS ARE ASSOCIATED WITH MOST NEOPLASIAS; AND (3) THAT CHROMOSOMAL REARRANGEMENTMAY PLAY A ROLE IN ONCOGENE ACTIVATION. HOWEVER, THE CURRENTLY USED NTP CHINESE HAMSTER OVARY (CHO) CHROMOSOMAL ABERRATION (CA) ASSAY LACKS SENSITIVITY AND, THUS, PRESENT CHO-CA RESULTS ARE OF LITTLE USE FOR EXAMINING THE MECH- ANISMS OF CLASTOGENESIS, MUTAGENESIS, NEOPLASIA, AND THEIR INTERRELATIONSHIPS. PHASE I RESEARCH WILL DEVELOP A NEW FRAGILE SITE CHO-CA ASSAY WHICH WILL INCLUDE: (1) PRE- TREATMENT OF CHO CELLS TO INDUCE SYNCHRONY AND ENHANCE CLASTOGENESIS AND TO MORE CLOSELY MIMIC IN CHO CELLS HUMAN SUBPOPULATIONS AT RISK FOR CHEMICALLY INDUCED NEOPLASIA; (2)USE OF A MORE INFORMATIVE PRETEST TO DETERMINE EFFECTIVE CONCENTRATIONS AND TO SELECT FIXATION TIMES BASED ON CHEMICALLY INDUCED CELL DELAY; AND (3) USE OF AUTOMATED SYSTEMS TO REDUCE CYTOGENETICIST TIME REQUIRED FOR EVALUA- TING THE RESULTS OF THE CA ASSAY. PHASE II RESEARCH WILL EVALUATE THE EFFECTS OF HIGH TOXICITY, HIGH SALT CONCENTRA- TIONS, OSMOLARITY, AND PH ON THE INDUCTION OF FRAGILE SITE- SPECIFIC CAS. AN EVALUATION OF THIS SYSTEM USING CODED COMPOUNDS WILL ALSO BE PERFORMED IN PHASE II.

* information listed above is at the time of submission.

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