MODIFIED MOUSE LYMPHOMA CELL FORWARD MUTATION ASSAY

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$267,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
4910
Agency Tracking Number:
4910
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
2224 Old Middlefield Way, Suite C, Mountain View, CA, 94043
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Ann D Mitchell
Principal Investigator
(415) 968-3534
Business Contact:
() -
Research Institute:
n/a
Abstract
TO ENHANCE THE USEFULNESS OF THE MOUSE LYMPHOMA ASSAY (MLA) FOR PREDICTING THE POSSIBLE OUTCOMES OF EXPOSURE TO CHEMICALS, SOME MLA PROCEDURES WILL BE EXAMINED TO DETERMINEWHETHER A MODIFIED ASSAY WOULD PERMIT GREATER SENSITIVITY AND EASE OF PERFORMANCE. PHASE I RESEARCH WILL INCLUDE: (1) AUTORADIOGRAPHY STUDIES TO DETERMINE IF THE PROCEDURES USED TO CLEANSE THE CULTURES OF TK-/-CELLS RENDER THE CELLS MORE SUSCEPTIBLE TO THE EFFECTS OF MUTAGENS, INDUCE A SYNCHRONOUS POPULATION OF CELLS THAT MAY BE PRESENT AT THE TIME OF CHEMICAL EXPOSURE, AND/OR INDUCE MUTAGENESIS AND CHROMOSOMAL ABERRATIONS; (2) MUTAGENESIS ASSAYS TO EVALUATE THE IMPACT OF VARYING THE EXPOSURE TIME TO DIFFERENT CHEMICAL CONCENTRATIONS; (3) USE OF AN AUTOMATED SYSTEM TO DETERMINE THE MOST PRECISE AND EFFICIENT METHOD FOR COUNTINGCELLS IN SUSPENSION; AND (4) EXPERIMENTS TO INVESTIGATE ASSAY MODIFICATIONS FOR CLONING CELLS IMMEDIATELY AFTER THE CLONING PERIOD. BASED ON THE OUTCOME OF PHASE I EXPERIMEN- TATION, A MODIFIED MLA WILL BE RECOMMENDED. IT WILL BE EVALUATED IN PHASE II BY ADDITIONAL RESEARCH AND THE TESTINGOF CODED COMPOUNDS. AS A RESULT OF THIS RESEARCH, THE MLA SHOULD BE MORE SENSITIVE, ECONOMICAL, AND PREDICTIVE OF GENOTOXICITY, WHICH SHOULD LEAD TO GREATER ACCEPTABILITY ANDUSE OF THE ASSAY IN INDUSTRIAL AND COMMERCIAL LABORATORIES.

* information listed above is at the time of submission.

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