MODIFIED MOUSE LYMPHOMA CELL FORWARD MUTATION ASSAY

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4910
Amount: $267,000.00
Phase: Phase II
Program: SBIR
Awards Year: 1987
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
2224 Old Middlefield Way, Suite C, Mountain View, CA, 94043
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Ann D Mitchell
 Principal Investigator
 (415) 968-3534
Business Contact
Phone: () -
Research Institution
N/A
Abstract
TO ENHANCE THE USEFULNESS OF THE MOUSE LYMPHOMA ASSAY (MLA) FOR PREDICTING THE POSSIBLE OUTCOMES OF EXPOSURE TO CHEMICALS, SOME MLA PROCEDURES WILL BE EXAMINED TO DETERMINEWHETHER A MODIFIED ASSAY WOULD PERMIT GREATER SENSITIVITY AND EASE OF PERFORMANCE. PHASE I RESEARCH WILL INCLUDE: (1) AUTORADIOGRAPHY STUDIES TO DETERMINE IF THE PROCEDURES USED TO CLEANSE THE CULTURES OF TK-/-CELLS RENDER THE CELLS MORE SUSCEPTIBLE TO THE EFFECTS OF MUTAGENS, INDUCE A SYNCHRONOUS POPULATION OF CELLS THAT MAY BE PRESENT AT THE TIME OF CHEMICAL EXPOSURE, AND/OR INDUCE MUTAGENESIS AND CHROMOSOMAL ABERRATIONS; (2) MUTAGENESIS ASSAYS TO EVALUATE THE IMPACT OF VARYING THE EXPOSURE TIME TO DIFFERENT CHEMICAL CONCENTRATIONS; (3) USE OF AN AUTOMATED SYSTEM TO DETERMINE THE MOST PRECISE AND EFFICIENT METHOD FOR COUNTINGCELLS IN SUSPENSION; AND (4) EXPERIMENTS TO INVESTIGATE ASSAY MODIFICATIONS FOR CLONING CELLS IMMEDIATELY AFTER THE CLONING PERIOD. BASED ON THE OUTCOME OF PHASE I EXPERIMEN- TATION, A MODIFIED MLA WILL BE RECOMMENDED. IT WILL BE EVALUATED IN PHASE II BY ADDITIONAL RESEARCH AND THE TESTINGOF CODED COMPOUNDS. AS A RESULT OF THIS RESEARCH, THE MLA SHOULD BE MORE SENSITIVE, ECONOMICAL, AND PREDICTIVE OF GENOTOXICITY, WHICH SHOULD LEAD TO GREATER ACCEPTABILITY ANDUSE OF THE ASSAY IN INDUSTRIAL AND COMMERCIAL LABORATORIES.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government