Genetic Engineering of Maize for Increased Tolerance to Heat Stress

Award Information
Agency: National Science Foundation
Branch: N/A
Contract: 0214789
Agency Tracking Number: 0214789
Amount: $99,990.00
Phase: Phase I
Program: STTR
Awards Year: 2002
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Genetic Archit Edu Analys
2308 6th Street, E., Brookings, SD, 57006
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Eugene Butler
 (605) 692-2751
 ebutler3@brookings.net
Business Contact
 Eugene Butler
Title: President
Phone: () -
Email: ebutler3@brookings.net
Research Institution
 U of South Dakota
 Zoran Ristic
 Biology Department
414 East Clark Street
Vermillion, SD, 57069
 (605) 677-6170
 Nonprofit college or university
Abstract
This Small Business Technology Transfer Research (STTR) Phase 1 Project proposes to develop transgenic maize (Zea mays L.) with increased tolerance to heat stress. It has recently discovered that a gene encoding the maize chloroplast protein synthesis elongation factor, EF-Tu, plays a role in the development of heat tolerance. EF-Tu protects other proteins from heat-induced inactivation and aggregation. The overall goal of this project is to genetically engineer maize that overproduces EF-Tu under high temperature conditions and to exploit the protective role that EF-Tu plays in heat tolerance. The key objectives of the Phase 1 research include (a) the creation of EF-Tu transgenic maize protoplasts, Arabidopsis and maize whole-plants and (b) the assessment of their heat tolerance in the laboratory. The results of the Phase 1 project will lay the groundwork for the follow-on Phase 2 and Phase 3 projects. They will include the testing the performance of EF-Tu transgenic maize in the field (Phase 2) and its commercialization (Phase 3).

* information listed above is at the time of submission.

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