Genetic Engineering of Maize for Increased Tolerance to Heat Stress

Award Information
Agency:
National Science Foundation
Branch
n/a
Amount:
$99,990.00
Award Year:
2002
Program:
STTR
Phase:
Phase I
Contract:
0214789
Award Id:
58288
Agency Tracking Number:
0214789
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
2308 6th Street, E., Brookings, SD, 57006
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Eugene Butler
(605) 692-2751
ebutler3@brookings.net
Business Contact:
Eugene Butler
President
() -
ebutler3@brookings.net
Research Institution:
U of South Dakota
Zoran Ristic
Biology Department
414 East Clark Street
Vermillion, SD, 57069
(605) 677-6170
Nonprofit college or university
Abstract
This Small Business Technology Transfer Research (STTR) Phase 1 Project proposes to develop transgenic maize (Zea mays L.) with increased tolerance to heat stress. It has recently discovered that a gene encoding the maize chloroplast protein synthesis elongation factor, EF-Tu, plays a role in the development of heat tolerance. EF-Tu protects other proteins from heat-induced inactivation and aggregation. The overall goal of this project is to genetically engineer maize that overproduces EF-Tu under high temperature conditions and to exploit the protective role that EF-Tu plays in heat tolerance. The key objectives of the Phase 1 research include (a) the creation of EF-Tu transgenic maize protoplasts, Arabidopsis and maize whole-plants and (b) the assessment of their heat tolerance in the laboratory. The results of the Phase 1 project will lay the groundwork for the follow-on Phase 2 and Phase 3 projects. They will include the testing the performance of EF-Tu transgenic maize in the field (Phase 2) and its commercialization (Phase 3).

* information listed above is at the time of submission.

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