MONOCLONAL ANTIBODIES AND IMMUNOASSAYS FOR CANNABINOIDS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 1557
Amount: $385,632.00
Phase: Phase II
Program: SBIR
Awards Year: 1986
Solitcitation Year: N/A
Solitcitation Topic Code: N/A
Solitcitation Number: N/A
Small Business Information
Genetic Diagnostics Corp.
160 Community Drive, Great Neck, NY, 11021
Duns: N/A
Hubzone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Harvey J. Brandwein
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
AS THE USE OF MARIHUANA HAS CONTINUED TO GROW, THE NEED FOR ACCURATE AND RELIABLE MEANS OF MEASURING CANNABINOID LEVELS HAS BECOME MORE URGENT. THIS PHASE I APPLICATION OUTLINES OUR INTEREST IN DEVELOPING SUCH A CANNABINOID ASSAY WHICH WILL BE RAPID, NON-ISOTOPIC, AND CAPABLE OF BEING PERFORMED IN A "FIELD" SETTING. OUR SPECIFIC GOALS FOR PHASE I WILL BE TO GENERATE MURINE MONOCLONAL ANTIBODIES TO THC, AND TO BEGIN WORK ON THE DEVELOPMENT OF A SENSITIVE AND VERSATILE THC FLUORESCENCE IMMUNOASSAY WHICH WILL BE FASTER, EASIER AND AT LEAST AS SENSITIVE AS CURRENT RADIOIMMUNOASSAYS, (DETECTS 10 NG/ML THC). MONOCLONAL ANTIBODIES WILL BE PREPARED AGAINST 2 DIFFERENT IMMUNOGENS. WE WILL FIRST USE A 5'-CARBOXY 9-THC-BSA CONJUGATE WHICH HAS BEEN GENEROUSLY OFFERED TO US BY NIDA. SUBSEQUENTLY, WE WILL ALSO USE A CONJUGATE WHICH WE PLAN TO PREPARE VIACARBOXYMETHYLATION AND CARBODIMIDE COUPLING OF THE PHENOLIC HYDROXYL OF 9-THC. APPROXIMATELY CHARACTERIZED ANTIBODIES WILL BE ATTACHED TO B-GAACTOSIDASE AND UTILIZED IN A RAPID SOLID-PHASE ENZYME IMMUNOASSAY USING FLUOROGENIC SUBSTRATES. SUCH AN ASSAY SYSTEM WHEN FULLY DEVELOPED, WILL HAVE A SIGNIFICANT COMMERCIAL MARKET, AND WILL BE USEFUL FOR LAW ENFORCEMENT AGENCIES, THE MILITARY, AND OTHER ASPECTS OF NIDA'S OVERALL RESEARCH PROGRAM.

* information listed above is at the time of submission.

Agency Micro-sites

US Flag An Official Website of the United States Government