MONOCLONAL ANTIBODIES AND IMMUNOASSAYS FOR CANNABINOIDS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$385,632.00
Award Year:
1986
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
1557
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Genetic Diagnostics Corp.
160 Community Drive, Great Neck, NY, 11021
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Harvey J. Brandwein
() -
Business Contact:
() -
Research Institution:
n/a
Abstract
AS THE USE OF MARIHUANA HAS CONTINUED TO GROW, THE NEED FOR ACCURATE AND RELIABLE MEANS OF MEASURING CANNABINOID LEVELS HAS BECOME MORE URGENT. THIS PHASE I APPLICATION OUTLINES OUR INTEREST IN DEVELOPING SUCH A CANNABINOID ASSAY WHICH WILL BE RAPID, NON-ISOTOPIC, AND CAPABLE OF BEING PERFORMED IN A "FIELD" SETTING. OUR SPECIFIC GOALS FOR PHASE I WILL BE TO GENERATE MURINE MONOCLONAL ANTIBODIES TO THC, AND TO BEGIN WORK ON THE DEVELOPMENT OF A SENSITIVE AND VERSATILE THC FLUORESCENCE IMMUNOASSAY WHICH WILL BE FASTER, EASIER AND AT LEAST AS SENSITIVE AS CURRENT RADIOIMMUNOASSAYS, (DETECTS 10 NG/ML THC). MONOCLONAL ANTIBODIES WILL BE PREPARED AGAINST 2 DIFFERENT IMMUNOGENS. WE WILL FIRST USE A 5'-CARBOXY 9-THC-BSA CONJUGATE WHICH HAS BEEN GENEROUSLY OFFERED TO US BY NIDA. SUBSEQUENTLY, WE WILL ALSO USE A CONJUGATE WHICH WE PLAN TO PREPARE VIACARBOXYMETHYLATION AND CARBODIMIDE COUPLING OF THE PHENOLIC HYDROXYL OF 9-THC. APPROXIMATELY CHARACTERIZED ANTIBODIES WILL BE ATTACHED TO B-GAACTOSIDASE AND UTILIZED IN A RAPID SOLID-PHASE ENZYME IMMUNOASSAY USING FLUOROGENIC SUBSTRATES. SUCH AN ASSAY SYSTEM WHEN FULLY DEVELOPED, WILL HAVE A SIGNIFICANT COMMERCIAL MARKET, AND WILL BE USEFUL FOR LAW ENFORCEMENT AGENCIES, THE MILITARY, AND OTHER ASPECTS OF NIDA'S OVERALL RESEARCH PROGRAM.

* information listed above is at the time of submission.

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