RECENT STUDIES IN MANY LABORATORIES HAVE DEMONSTRATED THE PRESENCE OF 2 UBIQUITOUS, CLOSELY RELATED ENZYMES, THE CALCIUM-ACTIVATED PROTEASES (CAP I AND II).

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$40,470.00
Award Year:
1985
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
3291
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Genetic Diagnostics Corpo
160 Community Drive, Great Neck, NY, 11021
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
H. BRANDWEIN
PRINCIPAL INVESTIGATOR
(516) 487-4711
Business Contact:
() -
Research Institution:
n/a
Abstract
RECENT STUDIES IN MANY LABORATORIES HAVE DEMONSTRATED THE PRESENCE OF 2 UBIQUITOUS, CLOSELY RELATED ENZYMES, THE CALCIUM-ACTIVATED PROTEASES (CAP I AND II). IN BRAIN, THE CAP ENZYMES ACT ON NEUROFILAMENT PROTEINS AND LEAD TO THE DEGRADATION OF THESE NEURONAL COMPONENTS. THE NEUROFILAMENT PROTEINS ARE ALSO OBSERVED TO BE STRUCTURALLY ALTERED IN THE BRAINS OF PATIENTS SUFFERING FROM ALZHEIMER'S DISEASE AND RELATED DEMENTIAS. THE GOAL OF THIS PROJECT IS TO EXAMINE IMMUNOCHEMICALLY THE POSSIBLE INVOLVEMENT OF CAP ACTIVITY IN THE NEURONAL LESIONS OF DEMENTIA, AND SEE IF ALTERED PROTEASE ACTIVITY THE PHASE I WORK WILL MAINLY INVOLVE THE PRODUCTION OF MONOCLONAL ANTIBODIES TO PURIFIED CAP I AND II BY HIGHLY EFFICIENT IMMUNIZATION, FUSION, AND SCREENING PROCEDURES. APPROPRIATE ANTIBODIES WILL THEN BE USED TO IMMUNOHISTOCHEMICALLY LOCALIZE THE ENZYMES IN BRAIN TISSUE TO SEE IF THE ENZYME (OR LOSS OF ENZYME) IS COINCIDENT WITH THE LESIONS (""PLAQUES'' AND ""TANGLES'') SEEN IN THE ALZHEIMER'S BRAIN. AT THE END OF PHASE I, WE WOULD EXPECT TO HAVE HIGH SPECIFIC ANTIBODIES AND BE DETERMINING THE ENZYMES' PRECISE LOCATION IN MAMMALIAN BRAIN. WE BELIEVE THIS WORK WILL PROVIDE IMPORTANT NEW RESEARCH TOOLS, AND MAY OFFER THE OPPORTUNITY FOR NEW DIAGNOSTIC (IN VIVO IMAGING) AND THERAPEUTIC (CAP MODIFICATION) APPROACHES TO ALZHEIMER'S DISEASE.

* information listed above is at the time of submission.

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