NOVEL IMMUNOGENS FOR AMPHETAMINE ENZYME IMMUNOASSAY

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 8135
Amount: $49,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1988
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Genetic Diagnostics Corp
160 Community Dr, Great Neck, NY, 11021
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 () -
Business Contact
Phone: () -
Research Institution
N/A
Abstract
DRUG ABUSE HAS CREATED A CONTINUING NEED FOR A RAPID AND ACCURATE SYSTEM FOR THE DETECTION OF AMPHETAMINES. THIS SBIR PROPOSAL ADDRESSES THE NEED FOR A RAPID AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR AMPHETAMINES WHICH CAN BE PERFORMED WITH ONLY MODEST LABORATORY FACILITIES. SINCE THE CRITICAL COMPONENT OF ANY IMMUNOASSAY IS THE AFFINITY AND SPECIFICITY OF THE ANTIBODY USED, WE PROPOSE TO GENERATE SPECIFIC MONOCLONAL ANTIBODIES FOR BOTH AMPHETAMINE AND METHAMPHETAMINE WHICH DO NOT DETECTVERY SIMILAR COMPOUNDS SUCH AS PHENYLPROPANOLAMINE, EPHEDRINE, AND PHENTERMINE WHICH ARE PRESENT IN MANY OVER THE COUNTER COLD, ALLERGY AND DIET MEDICATIONS. OUR SPECIFIC GOALS DURING THE PHASE I PERIOD WILL BE TO PREPARE SEVERAL "STERICALLY RIGID" AMPHETAMINE IMMUNOGENS, AND USE THEM TO GENERATE MONOCLONAL ANTIBODIES BY STANDARD HYBRIDOMA PROCEDURES. WE WILL CHARACTERIZE THESE ANTIBODIESFOR AFFINITY FOR AMPHETAMINE/METHAMPHETAMINE AND CROSS REACTIVITY WITH PHENYLPROPANOLAMINE, EPHEDRINE, AND PHENTERMINE. WE WILL ALSO INVESTIGATE THE NEW MONOCLONAL ANTIBODIES FOR USE IN POLYSTYRENE MICROTITER PLATE ELISA SYSTEMS, WHICH WE HAVE BEEN DEVELOPING FOR OTHER DRUGS OF ABUSE INCLUDING BENZODIAZEPINES, COCAINE, AND THC.

* information listed above is at the time of submission.

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