NOVEL IMMUNOGENS FOR AMPHETAMINE ENZYME IMMUNOASSAY

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$49,000.00
Award Year:
1988
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Award Id:
8135
Agency Tracking Number:
8135
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
160 Community Dr, Great Neck, NY, 11021
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
() -
Business Contact:
() -
Research Institute:
n/a
Abstract
DRUG ABUSE HAS CREATED A CONTINUING NEED FOR A RAPID AND ACCURATE SYSTEM FOR THE DETECTION OF AMPHETAMINES. THIS SBIR PROPOSAL ADDRESSES THE NEED FOR A RAPID AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR AMPHETAMINES WHICH CAN BE PERFORMED WITH ONLY MODEST LABORATORY FACILITIES. SINCE THE CRITICAL COMPONENT OF ANY IMMUNOASSAY IS THE AFFINITY AND SPECIFICITY OF THE ANTIBODY USED, WE PROPOSE TO GENERATE SPECIFIC MONOCLONAL ANTIBODIES FOR BOTH AMPHETAMINE AND METHAMPHETAMINE WHICH DO NOT DETECTVERY SIMILAR COMPOUNDS SUCH AS PHENYLPROPANOLAMINE, EPHEDRINE, AND PHENTERMINE WHICH ARE PRESENT IN MANY OVER THE COUNTER COLD, ALLERGY AND DIET MEDICATIONS. OUR SPECIFIC GOALS DURING THE PHASE I PERIOD WILL BE TO PREPARE SEVERAL "STERICALLY RIGID" AMPHETAMINE IMMUNOGENS, AND USE THEM TO GENERATE MONOCLONAL ANTIBODIES BY STANDARD HYBRIDOMA PROCEDURES. WE WILL CHARACTERIZE THESE ANTIBODIESFOR AFFINITY FOR AMPHETAMINE/METHAMPHETAMINE AND CROSS REACTIVITY WITH PHENYLPROPANOLAMINE, EPHEDRINE, AND PHENTERMINE. WE WILL ALSO INVESTIGATE THE NEW MONOCLONAL ANTIBODIES FOR USE IN POLYSTYRENE MICROTITER PLATE ELISA SYSTEMS, WHICH WE HAVE BEEN DEVELOPING FOR OTHER DRUGS OF ABUSE INCLUDING BENZODIAZEPINES, COCAINE, AND THC.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government