MAMMALIAN DNA SEQUENCE MAPPING CENTER

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$382,346.00
Award Year:
1989
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
4669
Agency Tracking Number:
4669
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
1335 Gateway Dr #2001, Melbourne, FL, 32904
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
John C Hozier
Principal Investigator
(305) 768-2048
Business Contact:
() -
Research Institution:
n/a
Abstract
BECAUSE OF THE RAPIDLY INCREASING USE OF IN SITU DNA SEQUENCE MAPPING TO MITOTIC CHROMOSOMES, A CENTER SHOULD BE ESTABLISHED TO DEVELOP THE MOST EFFICIENT TECHNIQUES AND TO AUTOMATE THE PROCEDURE. SUCH A CENTER WOULD MAKE AVAILABLE TO LABORATORIES INVOLVED IN THE CLONING OF DNA SEQUENCES THEABILITY TO EFFICIENTLY MAP TH SEQUENCES TO MAMMALIAN CHROMOSOMES. DNA SEQUENCING BY IN SITU HYBRIDIZATION WOULD THEREFORE BE MADE AVAILABLE TO LABORATORIES THAT DO NOT POSSESS THE TECHNICAL EXPERTISE IN HIGH-RESOLUTION CYTOGENETICS ESSENTIAL TO THIS MAPPING TECHNIQUE. CONDITIONS FOR IN SITU HYBRIDIZATION OF MOLECULAR PROBES TO MITOTIC CHROMOSOMES WOULD BE FURTHER DEVELOPED, FOCUSING ON IMPROVEMENTS IN PROBE LABELLING PROTOCOLS. COMPUTER- AUTOMATED PROCESSING FOR IN SITU HYBRIDIZATION AND AUTOMATEDDATA ACQUISITION AND ANALYSIS OF AUTORADIOGRAPHIC GRAIN DISTRIBUTIONS WOULD ALSO BE DEVELOPED. PHASE I ACTIVITY WILL BE CENTERED AROUND THE DEVELOPMENT OF COMPUTER PROGRAMSTO ANALYZE GRAIN DISTRIBUTIONS ON CHROMOSOMES AND THE STUDY OF THE AVAILABILITY OF COMPONENTS FOR MICRO-COMPUTER- CONTROLLED, AUTOMATED HYBRIDIZATION, AND AUTORADIOGRAPHIC PROCEDURES. THIS PHASE I ACTIVITY WOULD ALSO INCLUDE STUDIES OF THE OPTIMIZATION OF MOLECULAR PROBE LABELLING PROTOCOLS FOCUSING PARTICULARLY ON THE USE OF 35S-RNA LABELLING. FUTURE PHASE II ACTIVITIES WOULD INCLUDE CONSTRUCTION OF AN AUTOMATED IN SITU HYBRIDIZATION SYSTEM USING MICROCOMPUTER-CONTROLLED ROBOTICS TECHNOLOGY AND ENHANCEMENT OF THE COMPUTER ANALYSIS PROGRAMS TO PROVIDE ARCHIVING OF GENE SEQUENCE MAPPING DATA.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government