SYNTHESIS OF A SCA PROTEIN WITH ENZYMATIC ACTIVITY

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 13884
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1990
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Genex Corp
16020 Industrial Dr, Gaithersburg, MD, 20877
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Leslie S Johnson
 (301) 258-0552
Business Contact
Phone: () -
Research Institution
N/A
Abstract
A SINGLE-CHAIN ANTIBODY (SCA) PROTEIN WITH CATALYTIC ACTIVITY WILL BE PREPARED. THE SCA PROTEIN WILL BE DESIGNEDFROM THE VARIABLE REGION DEQUENCES OF ONE OF TWO ANTIBODIES FOUND TO HAVE LIPASE ACTIVITY, EITHER 2H6 OR 21H3. A GENE TO SYNTHESIZE THIS PROTEIN WILL BE CONSTRUCTED, AND THE PROTEIN WILL BE EXPRESSED IN ESCHERICHIA COLI. THE PROTEIN WILL BE RENATURED, PURIFIED, AND ASSAYED FOR ITS ENZYMATIC ACTIVITY. THIS SCA PROTEIN WILL BE ENGINEERED TO HAVE INCREASED STABILITY AND INCREASED CATALYTIC ACTIVITY DURING PHASE II OF THE PROJECT. THE SCA PROTEIN WITH CATALYTIC ACTIVITY PROVIDES THE MEANS TO ACCOMPLISH GENETIC CHANGES AND DETERMINE THE EFFECT ON CATALYTIC ACTIVITY AND STABILITY.

* information listed above is at the time of submission.

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