COMPARISON OF GENE MUTATION ASSAYS IN HUMAN LYMPHOBLASTOID A

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$150,000.00
Award Year:
1987
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Agency Tracking Number:
3155
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Gentest Limited Partnership
6 Henshaw Street, Woburn, MA, 01801
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Bruce W. Penman
Principal Investigator
(617) 935-5115
Business Contact:
() -
Research Institution:
n/a
Abstract
WE PROPOSE TO USE A GENE MUTATION ASSAY AT THE THYMIDINE KINASE LOCUS IN TK6 HUMAN LYMPHOBLASTS TO EVALUATE THE MUTAGENICITY OF CHEMICALS WHICH HAVE BEEN FOUND TO BE MUTAGENIC IN MOUSE LYMPHOMA L5178Y CELLS. THE TK6 CELL MUTATION ASSAY PROTOCOL HAS BEEN DESIGNED TO MINIMIZE DIFFERENCES WITH THE L5178Y CELL MUTATION ASSAY PROTOCOL AND YIELD COMPARABLE STATISTICAL POWER. THE RESULTS OBTAINED IN THE HUMAN LYMPHOBLAST MUTATION ASSAY SYSTEM WILL BE COMPARED TO THE TRTP MOUSE LYMPHOMA DATA BOTH QUALITATIVELY (DID THE CHEMICALS INDUCE A MUTAGENIC RESPONSE) AND QUANTITATIVELY (WHAT CONCENTRATION OF THE CHEMICAL WAS NECESSARY TO ELICIT A MUTAGENIC RESPONSE); CORRELATION BETWEEN THE TWO SYSTEMS WILL BE EXAMINED.

* information listed above is at the time of submission.

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