DEVELOPMENT OF SYSTEM TO DETECT PEROXISOME PROLIFERATORS

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 19226
Amount: $398,948.00
Phase: Phase II
Program: SBIR
Awards Year: 1993
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Gentest Corp.
6 Henshaw Street, Woburn, MA, 01801
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Bruce W Penman
 (617) 935-5115
Business Contact
Phone: () -
Research Institution
N/A
Abstract
WE WILL DEVELOP A NOVEL SYSTEM FOR DETECTING CHEMICALS WHICH INDUCE PEROXISOME PROLIFERATION. THIS SYSTEM WILL CONSIST OF A HUMAN CELL LINE WHICH HAS BEEN TRANSFECTED WITH SPECIFIC RECEPTOR AND REPORTER GENE CONSTRUCTS. CELLS WILL BE TRANSFECTED WITH CHIMERIC RECEPTORS CONTAINING THE LIGAND BINDING REGION OF THE PEROXISOME PROLIFERATOR RECEPTOR AND THE DNA BINDING REGION OF THE HUMAN GLUCOCORTICOID RECEPTOR. THE CNDA FOR THE REPORTER IS COUPLED TO A GLUCOCORTICOID RESPONSIVE PROMOTER. THE REPORTER GENE (CDNA) ENCODES AN ENZYME FOR WHICH THERE IS A DIRECT FLUOROMETRIC METHOD FOR DETERMINING CATALYTIC ACTIVITY. THE FLUOROMETRIC METHOD FOR DETERMINING CATALYTIC ACTIVITY. THE FLUOROMETRIC METHOD FOR CATALYTIC ACTIVITY IS SENSITIVE AND CAN BE PERFORMED IN WHOLE CELLS. THE RESULTING ASSAY SYSTEM SHOULD BE EASILY AMMENDABLE TO AUTOMATION FOR SCREENING LARGE NUMBERS OF CHEMICALS.

* information listed above is at the time of submission.

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