Development of Methods for Cytochrome P450 Production

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 22215
Amount: $48,689.00
Phase: Phase I
Program: SBIR
Awards Year: 1993
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Gentest Corp.
6 Henshaw Street, Woburn, MA, 01801
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Bruce W. Penman
 (617) 935-5115
Business Contact
Phone: () -
Research Institution
N/A
Abstract
We will develop and optimize methods for high level production of functional, catalytically active human cytochrome P450s by using baculovirus expression in insect cells. Approaches to maximize production of holoenzyme and minimize production of apoprotein will be developed and means to efficiently co-express cytochrome P450:NADPH oxidoreductase will be developed. Human cytochromes P4502A6 and P4502E1 will be used as prototype enzymes for this development. The eventual goal is to develop a complete panel expressing high levels of individual human cytochrome P450 forms. The commercial applications will be for the study of human xenobiotic metabolism.

* information listed above is at the time of submission.

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