Development of Caco-2 Cells Expressing Cytochromes P450

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: 1 R43 GM52270-01,
Agency Tracking Number: 29450
Amount: $489,598.00
Phase: Phase II
Program: SBIR
Awards Year: 1997
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
6 Henshaw Street, Woburn, MA, 01801
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 Charles Crespi
 () -
Business Contact
Phone: () -
Research Institution
We will evaluate the feasibility of expressing human cytochrome P450 (CYP) cDNAs in Caco-2 human inepithelial cells, a model system for the study of drug/xenobiotic uptake and transport. Expression susing CYP2A6 as a convenient and appropriate model enzyme and then extended to CYP3A4, the major cytin human intestinal epithelium. The suitability of transfected cells for studies of xenobiotic transThe overall goal is to develop Caco-2 cell derivatives which express the human cytochrome P450s founvivo in order to establish a better in vitro model system for drug transport / metabolism and for detherapeutically relevant drug-drug or drug-nutrient interactions.

* Information listed above is at the time of submission. *

Agency Micro-sites

SBA logo
Department of Agriculture logo
Department of Commerce logo
Department of Defense logo
Department of Education logo
Department of Energy logo
Department of Health and Human Services logo
Department of Homeland Security logo
Department of Transportation logo
Environmental Protection Agency logo
National Aeronautics and Space Administration logo
National Science Foundation logo
US Flag An Official Website of the United States Government