HETEROANTISERA AGAINST INTERFERON (IFN) AND INTERLEUKIN-2 (IL-2) ARE HIGHLY IMMUNOSUPPRESSIVE WHEN INJECTED INTO EXPERIMENTAL ANIMALS.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 3050
Amount: $49,535.00
Phase: Phase I
Program: SBIR
Awards Year: 1985
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
Genzyme Corpon
75 Kneeland Street, Boston, MA, 02111
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 DONALD S. MASTERS
 PRINCIPAL INVESTIGATOR
 (617) 451-1923
Business Contact
Phone: () -
Research Institution
N/A
Abstract
HETEROANTISERA AGAINST INTERFERON (IFN) AND INTERLEUKIN-2 (IL-2) ARE HIGHLY IMMUNOSUPPRESSIVE WHEN INJECTED INTO EXPERIMENTAL ANIMALS. THESE ANTICYTOKINES IMPAIR THE GENERATION OF CYTOLYTIC T CELLS, THE REJECTION OF TRANSPLANTED TUMORS, AND OTHER CELLULAR IMMUNE FUNCTIONS. MURINE MONOCLONAL ANTIBODIES AGAINST IL-2 HAVE BEEN AVAILABLE FOR SEVERAL YEARS, AND RECENTLY THE TECHNOLOGY FOR THE GENERATION OF HUMAN MONOCLONAL ANTIBODIES HAS EMERGED. PATIENTS TREATED WITH IFN IN VARIOUS CLINICAL TRIALS AND PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSIS WITH HIGH ENDOGENOUS LEVELS OF IFN DEVELOP ANTIBODIES AGAINST IFN. SIMILARLY, PATIENTS REPEATEDLY INJECTED WITH RECOMBINANT IL-2 AS TREATMENT FOR REFRACTORY CANCER DEVELOP IGG ANTIBODIES AGAINST IL-2. WE PROPOSE TO GENERATE HUMAN HYBRIDOMAS FROM THE B-LYMPHOCYTES OF THESE PATIENTS BY FUSION WITH HUMAN MYELOMA CELL LINES. THE CULTURE SUPERNATANTS OF THESE HYBRIDOMAS WILL BE SCREENED FOR ANTI-IL-2 ANTIBODIES WITH A SENSITIVE ELISA AND SUBSEQUENTLY FOR THE ABILITY TO INHIBIT IL-2-INDUCED T-CELL PROLIFERATION, CYTOLYTIC T-CELL INDUCTION, AND THE DEVELOPMENT OF A DELAYED-TYPE HYPERSENSITIVITY RESPONSE TO TUBERCULIN. THESE NEUTRALIZING ANTIBODIES MAY BE CLINICALLY USEFUL IN THE PREVENTION AND TREATMENT OF ORGAN ALLOGRAFT REJECTION AND IN THE

* information listed above is at the time of submission.

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