THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC A-D-MANNOSIDASE FROM HUMAN PLACENTAS AND BOVINE KIDNEYS.

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 4711
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1986
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
75 Kneeland Street, Boston, MA, 02111
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 SHIRISH HIRANI
 PRINCIPAL INVESTIGATOR
 (617) 451-1923
Business Contact
Phone: () -
Research Institution
N/A
Abstract
THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC A-D-MANNOSIDASE FROM HUMAN PLACENTAS AND BOVINE KIDNEYS. IT IS ANTICIPATED THAT BY EMPLOYING A COMBINATION OF CHROMATOGRAPHIC TECHNIQUES ON AFFINITY, HIGH-PERFORMANCE ANION EXCHANGE, AND/OR HIGH PERFORMANCE GEL FILTRATION MATRICES, HOMOGENEOUS ACIDIC A-D-MANNOSIDASE WILLBE OBTAINED. THIS WILL PERMIT THE GENERATION OF POLYCLONAL AND MONOCLONAL ANTIBODIES DURING PHASE II. FURTHERMORE, THEPURIFIED ENZYME AND ANTISERUM WILL PROVIDE A MEANS FOR CLONING THE GENE FOR ACIDIC A-D-MANNOSIDASE. THE ABSENCE OFTHIS ENZYME IN THE LYSOSOMAL STORAGE DISORDER, MANNOSIDOSIS,WHICH OCCURS IN BOTH MAN AND CATTLE, MAKES IT AN IDEAL CANDIDATE FOR STUDYING THE FEASIBILITY OF GENE REPLACEMENT THERAPY.

* Information listed above is at the time of submission. *

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