THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC A-D-MANNOSIDASE FROM HUMAN PLACENTAS AND BOVINE KIDNEYS.

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1986
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
4711
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Genzyme Corp
75 Kneeland Street, Boston, MA, 02111
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
SHIRISH HIRANI
PRINCIPAL INVESTIGATOR
(617) 451-1923
Business Contact:
() -
Research Institution:
n/a
Abstract
THE SPECIFIC AIM OF THIS PHASE I PROJECT IS TO PURIFY TO HOMOGENEITY ACIDIC A-D-MANNOSIDASE FROM HUMAN PLACENTAS AND BOVINE KIDNEYS. IT IS ANTICIPATED THAT BY EMPLOYING A COMBINATION OF CHROMATOGRAPHIC TECHNIQUES ON AFFINITY, HIGH-PERFORMANCE ANION EXCHANGE, AND/OR HIGH PERFORMANCE GEL FILTRATION MATRICES, HOMOGENEOUS ACIDIC A-D-MANNOSIDASE WILLBE OBTAINED. THIS WILL PERMIT THE GENERATION OF POLYCLONAL AND MONOCLONAL ANTIBODIES DURING PHASE II. FURTHERMORE, THEPURIFIED ENZYME AND ANTISERUM WILL PROVIDE A MEANS FOR CLONING THE GENE FOR ACIDIC A-D-MANNOSIDASE. THE ABSENCE OFTHIS ENZYME IN THE LYSOSOMAL STORAGE DISORDER, MANNOSIDOSIS,WHICH OCCURS IN BOTH MAN AND CATTLE, MAKES IT AN IDEAL CANDIDATE FOR STUDYING THE FEASIBILITY OF GENE REPLACEMENT THERAPY.

* information listed above is at the time of submission.

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