PURIFICATION OF HIGH MANNOSE OLIGOSACCHARIDES

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$500,000.00
Award Year:
1989
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
8040
Agency Tracking Number:
8040
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
75 Kneeland St, Boston, MA, 02111
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
(617) 876-9405
Business Contact:
() -
Research Institution:
n/a
Abstract
THE LONG-TERM OBJECTIVES OF THIS PROPOSAL ARE TO PREPARE PURE OLIGOSACCHARIDES FROM GLYCOPROTEINS POSSESSING ASPARAGINE-LINKED CARBOHYDRATE CHAINS AND TO USE THE OLIGOSACCHARIDES AND THEIR DERIVATIVES TO INVESTIGATE THE STRUCTURE, BIOSYNTHESIS AND FUNCTION OF THE GLYCAN CHAINS OF GLYCOPROTEINS. THE SPECIFIC AIMS OF THIS PROPOSAL ARE TOPURIFY AND CHARACTERIZE HIGH MANNOSE OLIGOSACCHARIDES FROM SOYBEAN PROTEINS. SOYBEAN AGGLUTININ AND 7S SOYBEAN PROTEINWILL BE PURIFIED, THEIR CARBOHYDRATE CHAINS RELEASED BY ENDO H AND N-GLYCANASE(R) DIGESTION, AND THE OLIGOSACCHARIDES ISOLATED AFTER CHROMATOGRAPHIC SEPARATION 2-AMINOPYRIDYL DERIVATIVES. THE OLIGOSACCHARIDES WILL BE CHARACTERIZED BY HPLC ANALYSIS, (1)H-MNR SPECTROSCOPY, METHYLATION ANALYSIS AND FAB MASS SPECTROSCOPY. THE PURIFIED OLIGOSACCHARIDES WILL BE SOLD TO THE RESEARCH COMMUNITY AS PART OF GENZYME'S GLYCOPROTEIN PRODUCT LINE. THE CARBOHYDRATES CAN BE USED AS STANDARDS FOR STRUCTURAL ANALYSIS, AS SUBSTRATES FOR ENZYMATIC REACTIONS, AND AS LIGANDS FOR EXAMINING THE ROLE OF THE GLYCANS IN THE BINDINGOF GROWTH FACTORS, HORMONES, TOXINS, VIRUSES, BACTERIA AND LECTINS TO CELLS. ONE POTENTIAL LARGE-SCALE APPLICATIONS FOR THE OLIGOSACCHARIDES IS AS A LIGAND TO TARGET THERAPEUTIC AGENTS TO MACROPHAGES.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government