Highly Sensitive Hand-Held Device for Quantitative Multiplex Protein Analysis Using Time-Resolved Assay

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$69,996.00
Award Year:
2009
Program:
SBIR
Phase:
Phase I
Contract:
W81XWH-09-C-0037
Award Id:
92019
Agency Tracking Number:
A083-173-0330
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
6 Davis Drive, Durham, NC, 27709
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
788103971
Principal Investigator:
Frank Wang
Director
(919) 883-5542
fwang@biomedomics.com
Business Contact:
Frank Wang
Director
(919) 883-5542
fwang@biomedomics.com
Research Institute:
n/a
Abstract
The point of care (POC) testing device is a very important tool for the Army in many different applications such as assessing the extent and effects of toxic and hazardous chemical exposure during deployments. Currently, a major challenge for POC testing technology is related to achieving multiplex capabilities with high detection sensitivity and quantification. In this proposal, we will apply our proprietary technologies in lateral flow immunoassay (LFIA), novel nanoparticles and time-resolved luminescent detection to technology development for a compact system for multiplex Lateral Flow Immunoassay (MLFIA). These proprietary technologies are well beyond the state of the art currently in practice, and are expected to increase test sensitivity by orders of magnitude as well as provide needed quantitation not available in today's LFIA tests. As with other POC testing devices, we will perform minimally invasive sampling of blood from fingers and utilize this small volume sample in MLFIA test strips read by our system. Time-resolved detection with long lifetime probes provides many significant advantages in sensitivity and practical uses since the detection signal is detected based on the signal's time-domain separation. This is very important for Army and clinical diagnostics applications, because the detection background noise coming from clinical samples and autofluorescence/scattering signals from strip materials are always issues for diagnostic sensitivity. At the end of the Phase I, we will provide a proof-of-concept demonstration of our MLFIA technology with of multiple well-known protein analytes.

* information listed above is at the time of submission.

Agency Micro-sites


SBA logo

Department of Agriculture logo

Department of Commerce logo

Department of Defense logo

Department of Education logo

Department of Energy logo

Department of Health and Human Services logo

Department of Homeland Security logo

Department of Transportation logo

Enviromental Protection Agency logo

National Aeronautics and Space Administration logo

National Science Foundation logo
US Flag An Official Website of the United States Government