Bioluminescent Detection of Chemical Agent Contamination

Award Information
Agency:
Department of Defense
Branch
Defense Advanced Research Projects Agency
Amount:
$374,947.00
Award Year:
2006
Program:
SBIR
Phase:
Phase II
Contract:
W31P4Q-06-C-0474
Award Id:
74339
Agency Tracking Number:
05SB2-0411
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
5750 Shier Rings Rd, Dublin, OH, 43016
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
001004258
Principal Investigator:
Dave Schofield
PI/Research Scientist
(843) 573-0095
dschofield@guildassociates.com
Business Contact:
Marcie Willem
Project Administrator
(614) 652-6003
marcie@guildassociates.com
Research Institution:
n/a
Abstract
Chemical weapons such as organophosphate (OP) nerve agents pose a deadly risk to military and civilian personnel since they are easily dispersed, they persist in the environment, and are extremely toxic. In addition, OP pesticide contamination causes 1 to 3 million unintentional poisonings annually worldwide. Consequently, there is an urgent need for natural biological systems that can detect and biodegrade these toxic compounds. This proposal will generate a Saccharomyces cerevisiae biocatalyst/biosensor that can detect and biodegrade OP agents such as VX, which is the most lethal and persistent OP. S. cerevisiae, which is a robust non-pathogenic microorganism that is resistant to environmental extremes, will be used as a self-contained biocatalyst/biosensor. Importantly, the yeast biosensor will also be able to detect the subsequent VX biodegraded products and will thereby provide a means for monitoring the decontamination process. The goal is to generate a biocatalyst/biosensor which: (i) will require minimal consumables; (ii) will be cheap to produce in bulk quantities; (iii) will emit distinct spectral properties following VX detection and biodegradation, and (iv) will be visually assessed using a simple hand held illumination device. The generation of a system that can detect and biodegrade OPs, and follow the decontamination process, is novel.

* information listed above is at the time of submission.

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