EXPRESSION OF DENGUE 3 ENVELOPE AND NONSTRUCTURAL 1 GLYCOPROTEINS IN THE FUNGUS NEUROSPORA CRASSA

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$67,723.00
Award Year:
1990
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
12551
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Hawaii Biotechnology Group Inc
99-193 Aiea Heights Dr, Aiea, HI, 96701
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Dr John M Ivy
(808) 486-5333
Business Contact:
() -
Research Institution:
n/a
Abstract
THE FOUR SEROTYPES OF DENGUE VIRUSES ARE A LEADING CAUSE OF MORBIDITY THROUGHOUT THE TROPICS AND SUBTROPICS. FOR THE DEVELOPMENT OF DIAGNOSTICS FOR AND SUBUNIT VACCINES AGAINST DENGUE VIRUS, A RELIABLE AND INEXPENSIVE SOURCE OF DENGUE PROTEINS IS NEEDED. THE EXPRESSION OF RECOMBINANT PROTEINS IN MICROORGANISMS OFFER SIGNAITICANT ADVANTAGES TO PURIFICATION OF NATIVE VIRAL PROTEINS. WE PROPOSE TO EVALUATE THE USE OF THE FUNGUS NEUROSPORA CRASSA TO PRODUCE AUTHENTIC DENGUE 3 ENVELOPE AND NONSTRUCTURAL 1 GLYCOPROTEINS.THE SEQUENCES ENCODING THESE PROTEINS WILL BE PLACED UNDER THE TRANSCRIPTIONAL CONTROL OF A CONSTITUTUVE NEUROSPORA PROMOTER, EITHER WITH THEIR OWN SECRETION SIGNAL PEPTIDES OR WITH A HETEROLOGOUS SIGNAL PEPTIDE. THE PROTEIN PRODUCED WILL BE EVALUATED WITH RESPECT TO MOLECULAR WEIGHT, GLYCOSYLATION AND ANTIGENICITY.

* information listed above is at the time of submission.

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