UREASE-LINED IMMUNOASSAY REAGENT STABILITY STUDIES

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$901,147.00
Award Year:
1992
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
15862
Agency Tracking Number:
15862
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Hawaii Biotechnology Gp Inc. (Currently HAWAII BIOTECH, INC.)
99-193 Aiea Heights Dr, Aiea, HI, 96701
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Dr T J G Raybould
Principal Investigator
(808) 486-5381
Business Contact:
() -
Research Institution:
n/a
Abstract
UREASE OFFERS DISTINCT ADVANTAGES OVER OTHER ENZYMES FOR PREPARATION OF CONJUGATES USED IN ENZYME-LINKED IMMUNO-SORBENT ASSAY SYSTEMS. MOLECULAR DEVICES CORPORATION (MENLO PARK, CA) HAS CAPITALIZED ON THESE ADVANTAGES IN THEIR THRESHOLD SYSTEM. THIS IMPRESSIVE SYSTEM UTILIZES AVIDIN-BIOTIN TO "CAPTURE" ANALYTE-BOUND ANTIBODY-UREASE CONJUGATE COMPLEXES ONTO A MEMBRANE, WHERE THEY ARE QUANTITATED BY A SENSOR THAT MONITORS THE FORMATION OF PRODUCT FROM THE UREASE-UREA REACTION. THE THRESHOLD SYSTEM IS OF INTEREST TO THE U.S. ARMY FOR A RANGE OF APPLICATIONS, BUT TO BE OF VALUE AS A FIELD DETECTOR, THE UREASE CONJUGATES REQUIRED MUST BE STABLE TO STORAGE AT ELEVATED TEMPERATURES FOR UP TO FIVE YEARS. METHODS WILL THEREFORE BE INVESTIGATED FOR PRODUCING HIGH ACTIVITY UREASE-ANTIBODY CONJUGATES THAT ARE STABLE TO SHORT TERM STORAGE AT 37 DEG C. PARAMETERS WILL THEN BE INVESTIGATED THAT AFFECT LYOPHILIZATION OF SELECTED UREASEANTIBODY CONJUGATES, AND THEIR STABILITY TO SHORT TERM STORAGE AT 60 DEG C. IT IS PROPOSED THAT ONE MONOCLONAL AND ONE POLYCLONAL MODEL SYSTEM ANTIBODY BE STUDIED. THE DATA OBTAINED FROM THESE PHASE I STUDIES WILL PROVIDE VALUABLE INFORMATION ON PROMISING AVENUES OF APPROACH FOR PHASE II, WHEN FURTHER REFINEMENTS WILL ENABLE PRODUCTION OF HIGH ACTIVITY, STABLE CONJUGATES, FOR USE IN U.S. ARMY APPLICATIONS OF THE THRESHOLD SYSTEM.

* information listed above is at the time of submission.

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