COFFEE ALPHA-GALACTOSIDASE CDNA CLONING FOR BLOOD PROCESSING

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1991
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
16691
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Hawaii Biotechnology Group, In
99-193 Aiea Heights Drive, Aiea, HI, 96701
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Ivy, John M
Principal Investigator
(808) 486-5333
Business Contact:
1 R43 HL46043-01
() -
Research Institution:
n/a
Abstract
IT HAS BEEN DEMONSTRATED THAT ALPHA-GALACTOSIDASE AND ALPHA-N-ACETYL GALACTOSAMINIDASE HAVE THE ABILITY TO ENZYMATICALLY CONVERT TYPE B AND A HUMAN ERYTHROCYTES, RESPECTIVELY, TO SEROTYPICALLY TYPE O ERYTHROCYTES. THIS ABILITY WOULD OBVIATE THE NEED FOR TYPING OR CROSSMATCHING, WOULD SIMPLIFY INVENTORY IN BLOOD BANKS, AND WOULD EXPEDITE THE DELIVERY OF TRANSFUSIONS. WE WILL SYNTHESIZE AND CLONE THE CDNA ENCODING THE ALPHA-GALACTOSIDASE FOUND IN GREEN COFFEE BEANS FOR EVENTUAL PRODUCTION IN MICROBIAL EXPRESSION SYSTEMS. DURING PHASE I, THIS WILL ENTAIL IDENTIFYING A COFFEE TISSUE TO USE AS A SOURCE OF THE ALPHA-GALACTOSIDASE MRNA, CDNA SYNTHESIS AND CLONING, AND IDENTIFYING RECOMBINANT CDNA CLONES EXPRESSING PROTEIN RECOGNIZED BY ANTI-ALPHA-GALACTOSIDASE ANTISERUM.

* information listed above is at the time of submission.

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