IMMUNOASSAYS FOR BRYOSTATINS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$483,000.00
Award Year:
1994
Program:
SBIR
Phase:
Phase II
Contract:
n/a
Award Id:
18989
Agency Tracking Number:
18989
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
99-193 Aiea Heights Drive, Aiea, HI, 96701
Hubzone Owned:
N
Minority Owned:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Paul G Grothaus
(808) 486-5333
Business Contact:
() -
Research Institute:
n/a
Abstract
THE OVERALL GOALS OF THIS PROJECT ARE TO DEVELOP IMMUNOASSAYS FOR THE DETECTION OF BRYOSTATIN 1 AND ITS ANALOGUES IN A VARIETY OF BIOLOGICAL MATRICES. THE BRYOSTATINS HAVE EXHIBITED STRONG ANTINEOPLASTIC ACTIVITY IN PRECLINICAL STUDIES AND ARE SCHEDULED TO ENTER PHASE I CLINICAL TRIALS LATER THIS YEAR. SENSITIVE ANALYTICAL METHODS ARE NEEDED FOR ESTABLISHING THE PROPER DOSING REGIMENS IN THESE STUDIES AND FOR MAXIMIZING YIELDS DURING THE LARGE SCALE ISOLATION OF THESE SCARCE NATURAL PRODUCTS. IN ADDITION, THE AVAILABLIITY OF BRYOSTATIN IMMUNOASSAYS WOULD FACILITATE BASIC RESEARCH EFFORTS TO DETERMINE THE BIODYNTHESIS AND ACTUAL SOURCE OF THESE INTERESTING COMPOUNDS. WE PLAN TO SYNTHESIZE WILL CHARACTERIZED BRYOSTATIN IMMUNOGENS WHICH SHOULD ELICIT ANTIBODY RESPONSES IN RABBITS AND MICE. THE GOAL OF PHASE I RESEARCH WILL BE TO DEMONSTRATE THE FEASIBILITY OF PRODUCING BRYOSTATIN IMMUNOASSAYS UTILIZING POLYCLONAL RABBIT ANTIBODIES. PHASE II RESEARCH WILL FOCUS ON REFINING THESE ASSAYS, PRODUCTION OF ADDITIONAL MONOCLONAL ANTIBODY-BASED IMMUNOASSAYS OF VARYING SPECIFICITIES FOR PARTICULAR APPLICATIONS, AND DEVELOPING PROCEDURES FOR DETERMINING BRYOSTATIN CONCENTRATIONS IN VARIOUS BIOLOGICAL MATRICES.

* information listed above is at the time of submission.

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