ORGANIC PHASE IMMUNOASSASY FOR CHLORINATED DIBENZODIOXINS

Award Information
Agency:
Department of Health and Human Services
Branch
n/a
Amount:
$50,000.00
Award Year:
1992
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
19218
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Hawaii Biotechnology Group Inc
99-193 Aiea Heights Drive, Aiea, HI, 96701
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
Gary S Bignami
(808) 486-5333
Business Contact:
() -
Research Institution:
n/a
Abstract
HIGH AFFINITY ANTIGEN-ANTIBODY BINDING IS ESSENTIALLY ANHYDROUS HYDROPHOBIC ORGANIC SOLVENTS HAS BEEN DEMONSTRATED IN OUR LABORATORY. USING AN ESTRADIOL-ANTIESTRADIOL SYSTEM, A PROTOTYPE ORGANIC PHASE RADIOIMMUNOASSAY (OPRIA) HAS BEEN DEVELOPED WHICH DETECTS 200 PG/ML OR ESTRADIOL IN A HEXANE MEDIUM CONTAINING LESS THAT 0.1% (W/W) WATER. THE RESEARCH WILL RESULT IN THE DEVELOPMENT OF AN OPRIA FOR POLYCHLORINATED DIBENZO-P-DIOXINS (PCDDS). THE ASSAY WILL EMPLOY THE MONOCLONAL ANTIBODIES DEVELOPED AT LAWRENCE LIVERMORE NATIONAL LABORATORY AN THE CROSS-REACTIVE ANTIGEN, [(3)H]1-N-ACETAMIDO-3,7,8-TRICHLORODIBENZO-P-DIOXIN([(3)H]ATRICDD). ATRICDD IS NOT A REGULATED HAZARDOUD WASTE, DUE TO ITS LOW TOXICITY. THE HYPOTHESIS THAT AN OPRIA FOR PCDDS WILL ALLOW DIRECT ANALYSIS OF THESE POLLUTANTS IN ORGANIC SOLVENT SAMPLE ETRACTS WILL BE TESTED. IF SUCCESSFUL, THIS TECHNOLOGY WILL SIGNIFICANTLY REDUCE THE COST AND TIME REQUIRED TO SCREEN WATER, SOIL, AND OTHER ENVIRONMENTAL SAMPLES POTENTIALLY CONTAMINATED WITH PCDDS.

* information listed above is at the time of submission.

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