Light-Regulated Heterologous Expression in Neurospora

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 22247
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Awards Year: 1993
Solicitation Year: N/A
Solicitation Topic Code: N/A
Solicitation Number: N/A
Small Business Information
99-193 Aiea Heights Drive, Aiea, HI, 96701
DUNS: N/A
HUBZone Owned: N
Woman Owned: N
Socially and Economically Disadvantaged: N
Principal Investigator
 John M. Ivy
 (808) 486-5333
Business Contact
Phone: () -
Research Institution
N/A
Abstract
Regulation of gene expression is critical for cellular and organismal development, and it often plays an important role in expression of heterologous recombinant proteins. Toward development of regulated expression vectors for filamentous fungi that will minimize materials cost and manipulation of the fermentation culture, we will define cis-acting, blue light responsive regulatory elements and to incorporate them into expression vectors. As a model, we will subclone sequences of the al-1 locus of Neurospora crassa and demonstrate that they will confer blue light regulation to promoters not normally exhibiting this regulation. Bovine chymosin will be used as the heterologous reporter sequence and gene product. If successfully developed, expression of heterologous products normally toxic to fungi could be prevented by fermentation in the dark and elicited by merely switching on illumination.

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