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Expression of Single-chain FV in Neurospora Crassa

Award Information
Agency: Department of Health and Human Services
Branch: N/A
Contract: N/A
Agency Tracking Number: 21929
Amount: $50,000.00
Phase: Phase I
Program: SBIR
Solicitation Topic Code: N/A
Solicitation Number: N/A
Solicitation Year: N/A
Award Year: 1993
Award Start Date (Proposal Award Date): N/A
Award End Date (Contract End Date): N/A
Small Business Information
99-193 Aiea Heights Drive
Aiea, HI 96701
United States
HUBZone Owned: No
Woman Owned: No
Socially and Economically Disadvantaged: No
Principal Investigator
 Eileen T. Nakano
 (808) 486-5333
Business Contact
Phone: () -
Research Institution

The filamentous fungi, owing to their ability to secrete high levels of proteins, are attractive organisms for the expression and secretion of heterologous proteins of commercial and medical value. Secretion is a key element in their attraction; secretion provides the opportunity for complex proteins to fold and be processed properly and secretion will facilitate purification. Proper folding and processing, simplified purification, and inexpensive culturing translate into less expensive production. We have demonstrated secretion and processing of an enzymatically active mammalian protein (preprochymosin) from Neurospora crassa, and we will test the feasibility of expressing a single chain antibody fragment in Neurospora. The use of Neurospora to express single chain antibodies is novel.

* Information listed above is at the time of submission. *

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