Expression of Antibodies in Neurospora

Award Information
Agency:
Department of Defense
Branch
Army
Amount:
$66,474.00
Award Year:
1994
Program:
SBIR
Phase:
Phase I
Contract:
n/a
Agency Tracking Number:
26303
Solicitation Year:
n/a
Solicitation Topic Code:
n/a
Solicitation Number:
n/a
Small Business Information
Hawaii Biotechnology Group,
99-193 Aiea Heights Drive, Aiea, HI, 96701
Hubzone Owned:
N
Socially and Economically Disadvantaged:
N
Woman Owned:
N
Duns:
n/a
Principal Investigator:
John Marshall Ivy, Ph.d.
(808) 486-5333
Business Contact:
() -
Research Institution:
n/a
Abstract
The filamentous fungi, owing to their ability to secrete high levels of proteins, are attractive organisms for the expression and secretion of heterologous proteins of commercial and medical value. Secretion is a key element in their attraction, for secretion enables proper folding and processing of complex proteins and facilitates their purification. Proper folding and processing, simplified purification, and inexpensive culturing will translate into less expensive production. We have demonstrated secretion and processing of an enzymatically active mammalian protein, bovine preprochymosin, from "Neurospora crassa." In the proposed Phase I effort, we will test the feasibility of expressing in "Neurospora" a single chain antibody fragment (scFv) of established medical value and determine the ability of the recombinant scFv to recognize tumor cell surface antigen.

* information listed above is at the time of submission.

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